Rabbit TCTP Polyclonal Antibody | anti-TPT1 antibody

TCTP, phosphorylated (Ser46) (Translationally controlled tumor protein, TCTP p23 HRF)

Cat. Num. AAA622361

• Gene Names: TPT1; TCTP; MGC128232
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: TCTP; Polyclonal Antibody; phosphorylated (Ser46) (Translationally controlled tumor protein; TCTP p23 HRF); Anti -TCTP; anti-TPT1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human TCTP protein at ~23kD only when phosphorylated at Ser46. In some cell types, may recognizes an 80-100kD protein of unknown origin.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-TPT1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:100 using citrate as an unmasking buffer.; Immunofluorescence (IF-IC): 1:200; Flow Cytometry: 1:100
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser46 of human TCTP protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from U-2 OS cells, untreated or synchronized in mitosis by thymidine block followed by release into nocodazole, using MBS622361.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda-phosphatase-treated (right), using MBS622361.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, control (left) or nocodazole-treated (right), using MBS622361.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells using MBS622361 compared to propidium iodide (DNA content).)

Immunofluorescence (IF)

(Confocal IF analysis of U-2 OS cells using MBS622361 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor is DRAQ5(fluorescent DNA dye).)

Related Product Information for anti-TPT1 antibody

Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, including a role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by Plk at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF and thus activating the TORC1 pathway, controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction of TCTP expression can cause tumor reversion (12).

Product Categories/Family for anti-TPT1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 62177164
• NCBI GeneID: 326599
• NCBI Accession #: NP_001014410.1
• NCBI GenBank Nucleotide #: NM_001014388.1
• UniProt Accession #: Q5E984; Q3SX12; Q862G3; Q862H4; Q862N1; Q862X2; Q862Y4; Q862L1
• Molecular Weight: 19,581 Da
• NCBI Official Full Name: translationally-controlled tumor protein
• NCBI Official Symbol: TPT1
• NCBI Official Synonym Symbols: TCTP; MGC128232
• NCBI Protein Information: translationally-controlled tumor protein
• UniProt Protein Name: Translationally-controlled tumor protein
• Protein Family: tRNA 2'-phosphotransferase
• UniProt Gene Name: TPT1
• UniProt Entry Name: TCTP_BOVIN

Uniprot Description

Function: Involved in calcium binding and microtubule stabilization

Subunit structure: Interacts with STEAP3

Subcellular location: Cytoplasm

Sequence similarities: Belongs to the TCTP family.