Rabbit 4E Binding Protein 1, non-phosphorylated Monoclonal Antibody | anti-EIF4EBP1 antibody

4E Binding Protein 1, non-phosphorylated (Thr46) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Cat. Num. AAA633422

• Gene Names: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Supernatant; Supernatant
• Synonyms: 4E Binding Protein 1; non-phosphorylated; Monoclonal Antibody; non-phosphorylated (Thr46) (Protein Synthesis Initiation Factor 4E Binding Protein; eIF-4EBP1; PHAS-1; PHAS-I; Eukaryotic Translation Initiation Factor BP); Anti -4E Binding Protein 1; anti-EIF4EBP1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 7H1
• Specificity: Detects endogenous levels of 4E-BP1 only when dephosphorylated at Thr46. Crossreacts with 4E-BP2 and 4E-BP3 dephosphorylated at equivalent sites.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

• Applicable Applications for anti-EIF4EBP1 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, ELISA, Western Blot.; Dilution: Western blot 1:1000; Flow Cytometry 1:200
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surrounding Thr46 of human 4E-BP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from COS cells, treated with λ phosphatase or 20% FBS as indicated, using Non-phospho-4E-BP1 (Thr46) (upper), Phospho-4E-BP1 (Thr37/46) Antibody (middle), and 4E-BP1 Antibody (lower).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of COS cells, untreated (blue) or λ phosphatase-treated (green), using Non-phospho-4E-BP1 (Thr46) compared to a nonspecific negative control antibody (red).)

Related Product Information for anti-EIF4EBP1 antibody

4E-BP1 (also known as PHAS-1) when bound to eIF4E, inhibits cap-dependent translation. Upon hyperphosphorylation of 4E-BP1 this interaction is disrupted and cap-dependent translation is activated (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4); while phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP-1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Product Categories/Family for anti-EIF4EBP1 antibody

Antibodies; Abs to Translation Initiation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 561630
• NCBI GeneID: 1978
• UniProt Accession #: Q13541; Q6IBN3; B2R502; D3DSW8
• Molecular Weight: 12,580 Da
• NCBI Official Full Name: 4E-binding protein 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E binding protein 1
• NCBI Official Symbol: EIF4EBP1
• NCBI Official Synonym Symbols: BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316
• NCBI Protein Information: eukaryotic translation initiation factor 4E-binding protein 1; OTTHUMP00000225572; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• Protein Family: Eukaryotic translation initiation factor 4E-binding protein
• UniProt Gene Name: EIF4EBP1
• UniProt Entry Name: 4EBP1_HUMAN

NCBI Description

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]

Uniprot Description

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; response to ethanol; TOR signaling pathway; cellular protein metabolic process; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; lung development; G1/S transition of mitotic cell cycle