SDS-Page
(Peptide Competition and Phosphatase Treatment Lysates prepared from A431 cells left unstimulated (1) or stimulated with EGF (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either not treated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at RT, and incubated with MBS627363 for two hours at RT in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2, 6), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5), After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that the peptide corresponding to Rac1/cdc42 [pS71] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
Rabbit anti-Human, Mouse Rac1 Polyclonal Antibody
Rac1, phosphorylated (Ser71)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Rac1; Polyclonal Antibody; phosphorylated (Ser71); Anti -Rac1; anti-Rac1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Rac1 when phosphorylated at Ser71. Species sequence homology: mouse, 100%. Cdc42 [pS71], 100% and RhoA/B/C [pS73], 92% are also expected to react.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Applicable Applications for anti-Rac1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000.
Dilution: Western Blot: 1:1000.
Immunogen
Synthetic phosphopeptide derived from a region of human Rac1-protein that contains serine 71.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
SDS-Page
(Peptide Competition and Phosphatase Treatment Lysates prepared from A431 cells left unstimulated (1) or stimulated with EGF (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either not treated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at RT, and incubated with MBS627363 for two hours at RT in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2, 6), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5), After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that the peptide corresponding to Rac1/cdc42 [pS71] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
SDS-Page
(Peptide Competition and Phosphatase Treatment Lysates prepared from A431 cells left unstimulated (1) or stimulated with EGF (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either not treated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at RT, and incubated with MBS627363 for two hours at RT in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2, 6), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5), After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that the peptide corresponding to Rac1/cdc42 [pS71] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
Related Product Information for anti-Rac1 antibody
Rac, cdc42 and Rho A, B and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP-bound Rac1 and cdc42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac1 is implicated in regulating serum response element (SRE), NFAT1 and nuclear factor kB (NF-kB) transcription activities. Activated Rac1 and cdc42 bind and activate PAK1, which in turn activates key downstream signaling proteins including MEKK-1 and JNK. Rac1 and cdc42 are phosphorylated on serine 71, a putative Akt site located between the proteinbinding domain and GTP-binding domain. Phosphorylation of Rac1 on serine 71 regulates its GTP-binding and GTPase activity.
Product Categories/Family for anti-Rac1 antibody
NCBI and Uniprot Product Information
Uniprot Description
Involved in axon outgrowth and myoblast fusion. Plays a role in regulating dorsal closure during embryogenesis. Involved in integrin alpha-PS3/beta-nu-mediated phagocytosis of Gram-positive S.aureus by hemocytes.
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Product Notes
The Rac1 (Catalog #AAA627363) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rac1, phosphorylated (Ser71) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rac1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the Rac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rac1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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