SDS-Page
(Peptide Competition Extracts prepared from PC12 cells treated with nocozadole were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with FAK [pS732] antibody for two hours at room temperature in a 1% Milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-serine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that only the peptide corresponding to FAK [pS732] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The phospho signal also was absent after lambda ( ) phosphatase stripping (data not shown), verifying that the antibody is phospho specific.)
Rabbit FAK Polyclonal Antibody
FAK, phosphorylated (Ser732) (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
Reactivity
Chicken, Frog, Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
FAK; Polyclonal Antibody; phosphorylated (Ser732) (Focal Adhesion Associated Protein Tyrosine Kinase; BC3); Anti -FAK; anti-FAK antibody
Host
Rabbit
Reactivity
Chicken, Frog, Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes mouse and rat FAK when phosphorylated at Ser732. Species sequence homology: human (100%), chicken (100%) and frog (90%).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.
Applicable Applications for anti-FAK antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide corresponding to human FAK that contains serine 732. Species sequence homology: mouse, rat and chicken.
Positive Control
PC12 cells treated with nocozadole; cdk5 +/- and cdk5 -/- mouse brain lysates.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
SDS-Page
(Peptide Competition Extracts prepared from PC12 cells treated with nocozadole were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with FAK [pS732] antibody for two hours at room temperature in a 1% Milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-serine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that only the peptide corresponding to FAK [pS732] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The phospho signal also was absent after lambda ( ) phosphatase stripping (data not shown), verifying that the antibody is phospho specific.)
SDS-Page
(Peptide Competition Extracts prepared from PC12 cells treated with nocozadole were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with FAK [pS732] antibody for two hours at room temperature in a 1% Milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho-serine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that only the peptide corresponding to FAK [pS732] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The phospho signal also was absent after lambda ( ) phosphatase stripping (data not shown), verifying that the antibody is phospho specific.)
Related Product Information for anti-FAK antibody
Focal Adhesion Kinase (FAK) is a 125kD non-receptor protein tyrosine kinase that plays a key role in signaling by growth factors, extracellular matrix and stress signals. Indeed, FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The serine/threonine kinase cyclin-dependent kinase-5 (Cdk5)-an important regulator in brain development-phosphorylates serine 732 of FAK, possibly regulating a centrosome-associated microtubule structure to promote nuclear translocation, a critical step in neuronal migration. This phosphorylation does not have a direct impact on the kinase activity of FAK, but appears to prevent the accumulation of FAK at the centrosome.
Product Categories/Family for anti-FAK antibody
Similar Products
Product Notes
The FAK (Catalog #AAA630226) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAK, phosphorylated (Ser732) (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) reacts with Chicken, Frog, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FAK can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the FAK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
