Rabbit S6 Ribosomal Protein Monoclonal Antibody | anti-rps6 antibody

S6 Ribosomal Protein, phosphorylated (Ser235/236)

Cat. Num. AAA632437

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: As reported
• Synonyms: S6 Ribosomal Protein; Monoclonal Antibody; phosphorylated (Ser235/236); Anti -S6 Ribosomal Protein; anti-rps6 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 8A30
• Specificity: Detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236.
• Purity/Purification: As reported
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-rps6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry.; Dilution: Western blot:1:2000; Immunohistochemistry: 1:50; Immunofluorescence: 1:100; Flow Cytometry: 1:25
• Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from PC12 and NIH/3T3 cells, treated with λ phosphatase, 20% FBS (20 min) or 100 ng/ml PDGF (20 min) as indicated, using MBS632437 S6 Phosphorylated Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb MBS632437 (lower).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MBS632437 S6 Phosphorylated Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide MBS632437(right).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, wortmannin and U0126 (blue), using MBS632437 S6 Phosphorylated Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb compared to a nonspecific negative control antibody (red).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (left) or 20% serum-treated (right), using MBS632437 S6 Ribosomal Phosphorylated protein (Ser235/Ser236) (D57.2.2E) XP™ Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye))

Related Product Information for anti-rps6 antibody

To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a small 19 amino acid region in the S6 carboxy terminus (4,5).

Product Categories/Family for anti-rps6 antibody

Antibodies; Abs to Ribosomal Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7649387
• UniProt Accession #: Q9M3V8
• Molecular Weight: 28,471 Da
• NCBI Official Full Name: S6 ribosomal protein
• UniProt Protein Name: 40S ribosomal protein S6
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: rps6
• UniProt Entry Name: RS6_ASPOF

Uniprot Description

Function: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA

Post-translational modification: Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes

Sequence similarities: Belongs to the ribosomal protein S6e family.