Testing Data
(Peptide Competition and Phosphatase Treatment: Lysates prepared from HeLa cells left untreated (1), treated with anisomycin (2), or TNF-alpha (3-7) were either left untreated (1-6) or treated with lambda (l) phosphatase (7). Lysates were then incubated with MAPKAPK-2 [pT334] antibody, following prior incubation with: no peptide (1-3, 7), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphothreonine-containing peptide (5) or, the phosphopeptide immunogen (6). The data show that only the peptide corresponding to MAPKAPK-2 [pT334] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific)
Rabbit anti-Human MAPKAP Kinase 2 Polyclonal Antibody | anti-MAPKAPK2 antibody
MAPKAP Kinase 2, phosphorylated (Thr334) (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1:1000
Testing Data
(Peptide Competition and Phosphatase Treatment: Lysates prepared from HeLa cells left untreated (1), treated with anisomycin (2), or TNF-alpha (3-7) were either left untreated (1-6) or treated with lambda (l) phosphatase (7). Lysates were then incubated with MAPKAPK-2 [pT334] antibody, following prior incubation with: no peptide (1-3, 7), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphothreonine-containing peptide (5) or, the phosphopeptide immunogen (6). The data show that only the peptide corresponding to MAPKAPK-2 [pT334] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific)
Testing Data
(Peptide Competition and Phosphatase Treatment: Lysates prepared from HeLa cells left untreated (1), treated with anisomycin (2), or TNF-alpha (3-7) were either left untreated (1-6) or treated with lambda (l) phosphatase (7). Lysates were then incubated with MAPKAPK-2 [pT334] antibody, following prior incubation with: no peptide (1-3, 7), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphothreonine-containing peptide (5) or, the phosphopeptide immunogen (6). The data show that only the peptide corresponding to MAPKAPK-2 [pT334] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific)
Testing Data
(Peptide Competition and Phosphatase Treatment Lysates prepared from HeLa cells left untreated (1), treated with anisomycin (2), or TNF-a (3-7) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-6) or treated with lambda (λ) phosphatase (7), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with MAPKAPK-2 [pT334] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1-3, 7), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphothreonine-containing peptide (5) or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that only the peptide corresponding to MAPKAPK-2 [pT334] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
Testing Data
(Peptide Competition and Phosphatase Treatment Lysates prepared from HeLa cells left untreated (1), treated with anisomycin (2), or TNF-a (3-7) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-6) or treated with lambda (λ) phosphatase (7), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with MAPKAPK-2 [pT334] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1-3, 7), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphothreonine-containing peptide (5) or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that only the peptide corresponding to MAPKAPK-2 [pT334] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
MAPKAPK2: a member of the MAPKAPK family of protein kinases. It is phosphorylated and activated by p38 in response to cytokines, stress and chemotactic factors.MAPKAPK-2 is apparently a direct target of p38 MAPK. HSP27 is one of its in vivo substrates. Two transcript variants encoding two different isoforms have been described.
Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; CAMK group; MAPKAPK family; MAPKAPK subfamily
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; signal transducer activity; ATP binding; protein kinase activity
Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; stress-activated MAPK cascade; leukotriene metabolic process; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of interleukin-6 production; response to stress; arachidonic acid metabolic process; inflammatory response; toll-like receptor 4 signaling pathway; inner ear development; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cytokine stimulus; Ras protein signal transduction; toll-like receptor signaling pathway; innate immune response; gene expression; regulation of tumor necrosis factor production; toll-like receptor 9 signaling pathway; G2/M transition DNA damage checkpoint; vascular endothelial growth factor receptor signaling pathway; response to DNA damage stimulus
Research Articles on MAPKAPK2
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Product Notes
The MAPKAPK2 mapkapk2 (Catalog #AAA626880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAPKAP Kinase 2, phosphorylated (Thr334) (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAPKAP Kinase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the MAPKAPK2 mapkapk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPKAP Kinase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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