Flow Cytometry (FC/FACS)
(Separation of K562 cells stained using anti-human Cyclin B1 (V152) PE antibody (10ul reagent per million cells in 100ul of cell suspension, red-filled) from K562 cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 6ug/ml, same as Cyclin B1 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of K562 cell suspension.)
Mouse anti-Mammalian Cyclin B1 Antibody | anti-CCNB1 antibody
Anti-Cyclin B1 PE
Reactivity
Mammalian
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Synonyms
Cyclin B1; Anti-Cyclin B1 PE; cyclin B1; anti-CCNB1 antibody
Host
Mouse
Reactivity
Mammalian
Isotype
Mouse IgG1
Clone Number
V152
Specificity
The mouse monoclonal antibody V152 recognizes cyclin B1, a 48kDa intracellular protein necessary for G2/M phase transition of the cell cycle.
Purity/Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
Applicable Applications for anti-CCNB1 antibody
Flow Cytometry (FC/FACS)(QC tested)
Application Notes
FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests. Intracellular staining.
Conjugation
PE
Immunogen
Recombinant His-tagged hamster cyclin B1
Excitation Laser
blue (488 nm)
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
Flow Cytometry (FC/FACS)
(Separation of K562 cells stained using anti-human Cyclin B1 (V152) PE antibody (10ul reagent per million cells in 100ul of cell suspension, red-filled) from K562 cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 6ug/ml, same as Cyclin B1 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of K562 cell suspension.)
Flow Cytometry (FC/FACS)
(Separation of K562 cells stained using anti-human Cyclin B1 (V152) PE antibody (10ul reagent per million cells in 100ul of cell suspension, red-filled) from K562 cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 6ug/ml, same as Cyclin B1 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of K562 cell suspension.)
Related Product Information for anti-CCNB1 antibody
Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lympHnode, testis et al.
References
Yang Y, Xue K, Li Z, Zheng W, Dong W, Song J, Sun S, Ma T, Li W: c-Myc regulates the CDK1/cyclin B1 dependent?G2/M cell cycle progression by histone H4 acetylation in Raji cells. Int J Mol Med. 2018 Jun;41(6):3366-3378., URL: https://www.ncbi.nlm.nih.gov/pubmed/29512702
Wolf F, Wandke C, Isenberg N, Geley S: Dose-dependent effects of stable cyclin B1 on progression through mitosis in human cells. EMBO J. 2006 Jun 21;25(12):2802-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/16724106
Balsara ZR, Misaghi S, Lafave JN, Starnbach MN: Chlamydia trachomatis infection induces cleavage of the mitotic cyclin B1. Infect Immun. 2006 Oct;74(10):5602-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/16988235
Geley S, Kramer E, Gieffers C, Gannon J, Peters JM, Hunt T: Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint. J Cell Biol. 2001 Apr 2;153(1):137-48., URL: https://pubmed.ncbi.nlm.nih.gov/11285280/
Chan YW, Chen Y, Poon RY: Generation of an indestructible cyclin B1 by caspase-6-dependent cleavage during mitotic catastrophe. Oncogene. 2009 Jan 15;28(2):170-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/18820706
Wolf F, Wandke C, Isenberg N, Geley S: Dose-dependent effects of stable cyclin B1 on progression through mitosis in human cells. EMBO J. 2006 Jun 21;25(12):2802-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/16724106
Balsara ZR, Misaghi S, Lafave JN, Starnbach MN: Chlamydia trachomatis infection induces cleavage of the mitotic cyclin B1. Infect Immun. 2006 Oct;74(10):5602-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/16988235
Geley S, Kramer E, Gieffers C, Gannon J, Peters JM, Hunt T: Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint. J Cell Biol. 2001 Apr 2;153(1):137-48., URL: https://pubmed.ncbi.nlm.nih.gov/11285280/
Chan YW, Chen Y, Poon RY: Generation of an indestructible cyclin B1 by caspase-6-dependent cleavage during mitotic catastrophe. Oncogene. 2009 Jan 15;28(2):170-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/18820706
Similar Products
Product Notes
The CCNB1 (Catalog #AAA1802268) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin B1 PE reacts with Mammalian and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin B1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS)(QC tested). FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests. Intracellular staining. Researchers should empirically determine the suitability of the CCNB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin B1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
