Mouse anti-Human CD369 Antibody | anti-CD369 antibody

Anti-Hu CD369 PE

Cat. Num. AAA1802269

• Gene Names: CLEC7A; BGR; CANDF4; DECTIN1; CLECSF12
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD369; Anti-Hu CD369 PE; Dectin-1; CLEC7A; BGR; CANDF4; SCARE2; C-type lectin domain containing 7A; anti-CD369 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Clone Number: 1500
• Specificity: The mouse monoclonal antibody 15E2 recognizes an extracellular epitope LWEDGSTFSSN of human CD369 (Dectin-1), a 33kDa transmembrane glycoprotein expressed predominantly on dendritic cells.
• Purity/Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide

• Applicable Applications for anti-CD369 antibody: Flow Cytometry (FC/FACS)(QC tested), Immunohistochemistry-Frozen (IHC-F)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests.
• Conjugation: PE
• Immunogen: Extracellular part of human CD369 with hIgG FC tag
• Excitation Laser: blue (488 nm)
• Workshop: HLDA X
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD369 (15E2) PE antibody (10ul reagent/100ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD369 (15E2) PE antibody (10ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD369 antibody

CD369 (dectin-1, beta-glucan receptor) is a 33kDa type II transmembrane glycoprotein of lectin family, and serves as a part of innate immunity system by binding to beta-glucan polymers, which are typical for yeast and mycobacterial cell walls. CD369 is expressed predominantly on dendritic cells, but it can be detected also on monocytes, macrophages, mast cells, eosinophils, B cells, endothelial cells, and sometimes also on some T cell subsets.

References

Ni L, Gayet I, Zurawski S, Duluc D, Flamar AL, Li XH, O'Bar A, Clayton S, Palucka AK, G Zurawski, Banchereau J, Oh SK: Concomitant activation and antigen uptake via human dectin-1 results in potent antigen-specific CD8+ T cell responses. J Immunol. 2010 Sep 15;185(6):3504-13, URL: https://pubmed.ncbi.nlm.nih.gov/20729328/?from_term=ni+l+gayet+i+zurawski+s&from_pos=2
Fischer M, Müller JP, Spies-Weisshart B, Gräfe C, Kurzai O, Hünniger K, Hochhaus A, Scholl S, Schnetzke U. Isoform localization of dectin-1 regulates the signaling quality of anti-fungal immunity. Eur J Immunol. 2017 May;47(5):848-859, URL: https://pubmed.ncbi.nlm.nih.gov/28303575/?from_term=scholl+s+hochhaus+a&from_pos=5
Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li XH, Hori T, Zurawski S, Liu YJ, Zurawski G, Oh S: Opposing roles of dectin-1 expressed on human plasmacytoid dendritic cells and myeloid dendritic cells in Th2 polarization. J Immunol. 2015 Aug 15;195(4):1723-31, URL: https://pubmed.ncbi.nlm.nih.gov/26123355/?from_term=zhang+w+xue+y&from_page=6&from_pos=10

NCBI and Uniprot Product Information

• NCBI GI #: 13384604
• NCBI GeneID: 64581
• NCBI Accession #: NP_072092.2
• NCBI GenBank Nucleotide #: NM_022570.4
• UniProt Accession #: Q9BXN2; Q6IPS7; Q96D32; Q96DR9; Q96LD3; Q96PA4; Q96PA5; Q96PA6; B2R861; B7Z494; B7Z5A9; B7Z5B9
• Molecular Weight: 27,627 Da
• NCBI Official Full Name: C-type lectin domain family 7 member A isoform b
• NCBI Official Synonym Full Names: C-type lectin domain family 7, member A
• NCBI Official Symbol: CLEC7A
• NCBI Official Synonym Symbols: BGR; CANDF4; DECTIN1; CLECSF12
• NCBI Protein Information: C-type lectin domain family 7 member A; dectin-1; beta-glucan receptor; lectin-like receptor 1; DC-associated C-type lectin 1; C-type lectin superfamily member 12; dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; C-typ
• UniProt Protein Name: C-type lectin domain family 7 member A
• UniProt Gene Name: CLEC7A
• UniProt Synonym Gene Names: BGR; CLECSF12; DECTIN1; DC-associated C-type lectin 1; Dectin-1
• UniProt Entry Name: CLC7A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: cytoplasm; plasma membrane; integral to membrane

Molecular Function: metal ion binding; carbohydrate binding; MHC protein binding; pattern recognition receptor activity

Biological Process: T cell activation; innate immune response; carbohydrate mediated signaling; phagocytosis, recognition; cell recognition; pattern recognition receptor signaling pathway; defense response to protozoan; inflammatory response

Disease: Candidiasis, Familial, 4; Aspergillosis, Susceptibility To