Mouse anti-Human CD95 Antibody | anti-CD95 antibody

Anti-Hu CD95 PE-DyLight 594

Cat. Num. AAA1802275

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-DyLight594 (PE-DyLight594) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD95; Anti-Hu CD95 PE-DyLight 594; FAS1; APT1; APO-1; FASTM; ALPS1A; TNFRSF6; Fas cell surface death receptor; anti-CD95 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1
• Clone Number: LT95
• Specificity: The antibody LT95 reacts with an extracellular epitope on CD95 (Fas/APO-1), a 46kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
• Purity/Purification: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-DyLight594 (PE-DyLight594) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide

• Applicable Applications for anti-CD95 antibody: Flow Cytometry (FC/FACS)(QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (0.4ml) is sufficient for 100 tests.
• Conjugation: PE-DyLight 594
• Immunogen: HUT-78 human T cell lymphoma cell line
• Excitation Laser: blue (488 nm)
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD95 (LT95) PE-DyLight 594 antibody (4ul reagent/100ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD95 (LT95) PE-DyLight 594 antibody (4ul reagent/100ul of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10ul reagent/100ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD95 positive T cells (red-filled) from CD95 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD95 (LT95) PE-DyLight 594 antibody (4ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD95 antibody

CD95 (Fas, APO-1), a 46kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.

References

Drosopoulos KG, Robertsml, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A.: Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway. J Biol Chem. 2005 Jun 17;280(24):22856-67. Epub 2005 Mar 8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15757891?dopt=
Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314(3):554-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/18078929?ordin
Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines. Blood. 2005 Sep 15;106(6):2033-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/15941911?ordin
Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol. 2003 Jun 15;170(12):6209-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/12794152?ordin
Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998 Mar 16;17(6):1675-87., URL: https://www.ncbi.nlm.nih.gov/pubmed/9501089?ordina