Typical Testing Data/Standard Curve (for reference only)
Human Breakpoint cluster region protein (BCR) ELISA Kit | BCR elisa kit
Human Breakpoint cluster region protein (BCR) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BCR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BCR antibody and an BCR-HRP conjugate. The assay sample and buffer are incubated together with BCR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCR concentration since BCR from samples and BCR-HRP conjugate compete for the anti-BCR antibody binding site. Since the number of sites is limited, as more sites are occupied by BCR from the sample, fewer sites are left to bind BCR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Bcr: a protein with serine/threonine protein kinase activity and is a GTPase-activating protein (GAP) for RAC1 and CDC42. The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. A breakpoint cluster region protein that participates in a t(9;22)(q34;q11) chromosomal translocation that produces a BCR-ABL oncogene responsible for chronic myeloid leukemia (CML), acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator. Tyr177 of human Bcr, phosphorylated in the Bcr-Abl fusion protein, provides a docking site for Gab2 and GRB2, and is important in the transforming activity of Bcr-Abl. Sequence variants of the Bcr protein may be associated with bipolar disorder. Two alternatively spliced isoforms of the human protein have been reported.
Protein type: Protein kinase, Ser/Thr (non-receptor); GAPs; EC 2.7.11.1; Protein kinase, atypical; GEFs, Rac/Rho; GAPs, Rac/Rho; Kinase, protein; Oncoprotein; ATYPICAL group; BCR family
Chromosomal Location of Human Ortholog: 22q11.23
Cellular Component: postsynaptic membrane; protein complex; membrane; postsynaptic density; cytosol; cell junction
Molecular Function: protein serine/threonine kinase activity; Rho guanyl-nucleotide exchange factor activity; protein binding; enzyme binding; protein-tyrosine kinase activity; kinase activity; ATP binding; GTPase activator activity
Biological Process: inner ear morphogenesis; peptidyl-tyrosine phosphorylation; regulation of cell cycle; protein amino acid autophosphorylation; response to lipopolysaccharide; platelet-derived growth factor receptor signaling pathway; signal transduction; protein amino acid phosphorylation; negative regulation of neutrophil degranulation; regulation of small GTPase mediated signal transduction; positive regulation of phagocytosis; small GTPase mediated signal transduction; negative regulation of inflammatory response; brain development; actin cytoskeleton organization and biogenesis; neuromuscular process controlling balance; negative regulation of cell migration
Disease: Leukemia, Acute Lymphoblastic; Leukemia, Chronic Myeloid
Research Articles on BCR
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Product Notes
The Human BCR bcr (Catalog #AAA7213758) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7213758 ELISA Kit recognizes Human BCR. It is sometimes possible for the material contained within the vial of "Breakpoint cluster region protein (BCR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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