Lewis Rat anti-Human CD77 Monoclonal Antibody | anti-CD77 antibody

Anti-Hu CD77 PE

Cat. Num. AAA1801915

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD77; Monoclonal Antibody; Anti-Hu CD77 PE; globotriaosylceramide Gb3; Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer; P blood group; anti-CD77 antibody

• Host: Lewis Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Lewis Rat IgM
• Clone Number: 38.13
• Specificity: The rat monoclonal antibody 38.13 recognizes CD77 (globotriaosylceramide Gb3), a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt's lymphoma cells.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing Tris buffered saline (TBS) solution with 15mM sodium azide

• Applicable Applications for anti-CD77 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Conjugation: PE
• Immunogen: Daudi cell line (Burkitt's lymphoma)
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Separation of RAJI cells stained using anti-human CD77 (38.13) PE antibody (10 ul reagent per million cells in 100 ul of cell suspension, red-filled) from RAJI cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 5 ug/ml, same as CD77 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of RAJI cell suspension.)

Related Product Information for anti-CD77 antibody

CD77 (globotriaosylceramide Gb3), also known as the Pk blood group antigen, BLA (Burkitt's lymphoma associated antigen), or CTH (ceramide trihexoside) is a neutral glycosphingolipid composed of three carbohydrate molecules linked to a lipid moiety in the cell membrane (Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer). It is expressed on germinal center B cells, Burkitt's lymphoma cells, it can be induced on extrafolicular B cells and it is also found on endothelia and epithelia. CD77 may be involved in elimination of germinal center B cells that fail to produce high affinity antibodies, and serves also as receptor for shiga toxin and verotoxin.

References

*Högerkorp CM, Borrebaeck CA: The human CD77- B cell population represents a heterogeneous subset of cells comprising centroblasts, centrocytes, and plasmablasts, prompting phenotypical revision. J Immunol. 2006 Oct 1;177(7):4341-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/16982868,*Senda M, Ito A, Tsuchida A, Hagiwara T, Kaneda T, Nakamura Y, Kasama K, Kiso M, Yoshikawa K, Katagiri Y, Ono Y, Ogiso M, Urano T, Furukawa K, Oshima S, Furukawa K: Identification and expression of a sialyltransferase responsible for the synthesis of disialylgalactosylgloboside in normal and malignant kidney cells: downregulation of ST6GalNAc VI in renal cancers. Biochem J. 2007 Mar 15;402(3):459-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=senda+m+,*Taga S, Carlier K, Mishal Z, Capoulade C, Mangeney M, Lécluse Y, Coulaud D, Tétaud C, Pritchard LL, Tursz T, Wiels J: Intracellular signaling events in CD77-mediated apoptosis of Burkitt's lymphoma cells. Blood. 1997 Oct 1;90(7):2757-67., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=taga+s+c,*Wiels J, Fellous M, Tursz T: Monoclonal antibody against a Burkitt lymphoma-associated antigen. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6485-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/7031655