Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CLEC2 (AYP1) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Mouse anti-Human CLEC2 Antibody | anti-CLEC2 antibody
Anti-Hu CLEC2 PE
Gene Names
CLEC1B; CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Synonyms
CLEC2; Anti-Hu CLEC2 PE; CLEC1B; CLEC2B; PRO1384; QDED721; C-type lectin domain family 1 member B; anti-CLEC2 antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG1 kappa
Clone Number
AYP1
Specificity
The mouse monoclonal antibody AYP1 recognizes an epitope within the extracellular part of CLEC2, a transmembrane glycoprotein expressed on activated plateles and on platelet microparticles.
Purity/Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
Applicable Applications for anti-CLEC2 antibody
Flow Cytometry (FC/FACS)(QC tested)
Application Notes
FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests.
Conjugation
PE
Immunogen
A recombinant extracellular domain of human CLEC2 (amino acids 68-229)
Excitation Laser
blue (488 nm)
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CLEC2 (AYP1) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CLEC2 (AYP1) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Flow Cytometry (FC/FACS)
(Separation of human thrombocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CLEC2 (AYP1) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Flow Cytometry (FC/FACS)
(Separation of human thrombocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CLEC2 (AYP1) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Related Product Information for anti-CLEC2 antibody
CLEC2 (C-type lectin-like receptor 2) functions as a platelet receptor for the lymphatic endothelial marker, PDPN, and mediates platelet activation. Besides platelets, it can be found on myeloid cells and NK cells. CLEC2 functions also as an attachment factor for HIV-1 and facilitates its capture by platelets. Platelet-aggregating snake venom protein rhodocytin also binds to CLEC2.
References
Gitz E, Pollitt AY, Gitz-Francois JJ, Alshehri O, Mori J, Montague S, Nash GB, Douglas MR, Gardiner EE, Andrews RK, Buckley CD, Harrison P, Watson SP: CLEC-2 expression is maintained on activated platelets and on platelet microparticles. Blood 2014 Oct 2;124(14):2262-70, URL: https://pubmed.ncbi.nlm.nih.gov/25150298/
Sung PS, Huang TF, Hsieh SL: Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2. Nat Commun. 2019 Jun 3;10(1):2402, URL: https://pubmed.ncbi.nlm.nih.gov/31160588/
Sung PS, Huang TF, Hsieh SL: Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2. Nat Commun. 2019 Jun 3;10(1):2402, URL: https://pubmed.ncbi.nlm.nih.gov/31160588/
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,096 Da
NCBI Official Full Name
C-type lectin domain family 1 member B isoform b
NCBI Official Synonym Full Names
C-type lectin domain family 1 member B
NCBI Official Symbol
CLEC1B
NCBI Official Synonym Symbols
CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
NCBI Protein Information
C-type lectin domain family 1 member B
UniProt Protein Name
C-type lectin domain family 1 member B
UniProt Gene Name
CLEC1B
UniProt Synonym Gene Names
CLEC2; CLEC-2
UniProt Entry Name
CLC1B_HUMAN
Uniprot Description
CLEC1B: Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. Acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 12p13.2
Cellular Component: plasma membrane
Molecular Function: protein binding; transmembrane receptor activity
Biological Process: cell surface receptor linked signal transduction; defense response; platelet activation
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Product Notes
The CLEC2 clec1b (Catalog #AAA1802273) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CLEC2 PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLEC2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS)(QC tested). FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests. Researchers should empirically determine the suitability of the CLEC2 clec1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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