Flow Cytometry (FC/FACS)
(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307d (A1) PE antibody (10ul reagent/100ul of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (4ul reagent/100ul of peripheral whole blood).)
Mouse anti-Human CD307d Antibody | anti-CD307d antibody
Anti-Hu CD307d PE
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Synonyms
CD307d; Anti-Hu CD307d PE; FcRL4; FCRH4; IRTA1; Fc receptor like 4; anti-CD307d antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG2a kappa
Clone Number
A1
Specificity
The mouse monoclonal antibody A1 recognizes an epitope within extracellular domain of CD307d, a transmembrane glycoprotein expressed mainly on memory B cells.
Purity/Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
Applicable Applications for anti-CD307d antibody
Flow Cytometry (FC/FACS)(QC tested)
Application Notes
FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests.
Conjugation
PE
Immunogen
DNA-immunization followed by a boost with CD307d-transfected cells
Excitation Laser
blue (488 nm)
Workshop
HLDA IX
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
Flow Cytometry (FC/FACS)
(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307d (A1) PE antibody (10ul reagent/100ul of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (4ul reagent/100ul of peripheral whole blood).)
Flow Cytometry (FC/FACS)
(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307d (A1) PE antibody (10ul reagent/100ul of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (4ul reagent/100ul of peripheral whole blood).)
Related Product Information for anti-CD307d antibody
CD307d is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITIM motifs and one ITSM motif in its cytoplasmic domain. CD307d is expressed mainly on the surface of memory B cells in mucosa-associated lymphoid tissues. It binds to aggregated immunoglobulin molecules (IgA, IgG). Defects of CD307d may play a role in HIV-induced memory B cell dysfunction.
References
Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26, URL: https://www.ncbi.nlm.nih.gov/pubmed/19494275
Similar Products
Product Notes
The CD307d (Catalog #AAA1802264) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD307d PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD307d can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS)(QC tested). FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests. Researchers should empirically determine the suitability of the CD307d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD307d, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
