Mouse C5aR2 Antibody | anti-C5aR2 antibody

Anti-Hu C5aR2 PE

Cat. Num. AAA1802271

• Gene Names: C5AR2; C5L2; GPF77; GPR77
• Reactivity: Human, Non-human primates
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: C5aR2; Anti-Hu C5aR2 PE; C5L2; GPR77; GPF77; complement component 5a receptor 2; anti-C5aR2 antibody

• Host: Mouse
• Reactivity: Human, Non-human primates
• Isotype: Mouse IgG2a kappa
• Clone Number: 1D9-M12
• Specificity: The mouse monoclonal antibody 1D9-M12 recognizes an extracellular epitope on C5aR2 (C5L2), a C5a complement receptor, which is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages.
• Purity/Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide

• Applicable Applications for anti-C5aR2 antibody: Flow Cytometry (FC/FACS)(QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests.
• Conjugation: PE
• Immunogen: L1.2 cells transfected with human C5aR2
• Excitation Laser: blue (488 nm)
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) PE antibody (10ul reagent/100ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human neutrophil granulocytes (red-filled) from C5aR2 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) PE antibody (10ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-C5aR2 antibody

C5aR2, also known as C5L2, is one of two receptors for C5a (anaphylatoxin). It is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages, and seems to have both pro-inflammatory and anti-inflammatory roles, depending on circumstances. Unlike CD88, C5aR2 is not coupled to G-protein, thus the modulatory role is more likely.

References

Muenstermann M, Strobel L, Klos A, Wetsel RA, Woodruff TM, Köhl J, Johswich KO: Distinct roles of the anaphylatoxin receptors C3aR, C5aR1 and C5aR2 in experimental meningococcal infections. Virulence 2019 Dec;10(1):677-694., URL: https://pubmed.ncbi.nlm.nih.gov/31274379/
Fonseca MI, McGuire SO, Counts SE, Tenner AJ: Complement activation fragment C5a receptors, CD88 and C5L2, are associated with neurofibrillary pathology. J Neuroinflammation 2013, Feb 8;10:25, URL: https://pubmed.ncbi.nlm.nih.gov/23394121/
An LL, Gorman JV, Stephens G, Swerdlow B, Warrener P, Bonnell J, Mustelin T, Fung M, Kolbeck R: Complement C5a induces PD-L1 expression and acts in synergy with LPS through Erk1/2 and JNK signaling pathways. Sci Rep. 2016 Sep 14;6:33346., URL: https://pubmed.ncbi.nlm.nih.gov/27624143/
Spaan AN, Vrieling M, Wallet P, Badiou C, Reyes-Robles T, Ohneck EA, Benito Y, de Haas CJ, Day CJ, Jennings MP, Lina G, Vandenesch F, van Kessel KP, Torres VJ, van Strijp JA, Henry T: The staphylococcal toxins gamma-haemolysin AB and CB differentially target phagocytes by employing specific chemokine receptors. Nat Commun. 2014 Nov 11;5:5438., URL: https://pubmed.ncbi.nlm.nih.gov/25384670/
Hao J, Wang C, Yuan J, Chen M, Zhao MH: A pro-inflammatory role of C5L2 in C5a-primed neutrophils for ANCA-induced activation. PLoS One, 2013, Jun 13;8(6):e66305., URL: https://pubmed.ncbi.nlm.nih.gov/23785491/

NCBI and Uniprot Product Information

• NCBI GI #: 8923873
• NCBI GeneID: 27202
• NCBI Accession #: NP_060955.1
• NCBI GenBank Nucleotide #: NM_018485.2
• UniProt Accession #: Q9P296; B2RA09
• Molecular Weight: 36,080 Da
• NCBI Official Full Name: C5a anaphylatoxin chemotactic receptor 2
• NCBI Official Synonym Full Names: complement component 5a receptor 2
• NCBI Official Symbol: C5AR2
• NCBI Official Synonym Symbols: C5L2; GPF77; GPR77
• NCBI Protein Information: C5a anaphylatoxin chemotactic receptor 2; G protein-coupled receptor 77; G protein-coupled receptor C5L2; C5a anaphylatoxin chemotactic receptor C5L2
• UniProt Protein Name: C5a anaphylatoxin chemotactic receptor 2
• Protein Family: C5a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C5AR2
• UniProt Synonym Gene Names: C5L2; GPR77
• UniProt Entry Name: C5AR2_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Uniprot Description

C5AR2: Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: apical part of cell; integral to membrane; plasma membrane; basal plasma membrane

Molecular Function: protein binding; C5a anaphylatoxin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of tumor necrosis factor production; inflammatory response; chemotaxis; positive regulation of epithelial cell proliferation