Mouse anti-Human CD66c Antibody | anti-CD66c antibody

Anti-Hu CD66c PE

Cat. Num. AAA1802265

• Gene Names: CEACAM6; NCA; CEAL; CD66c
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD66c; Anti-Hu CD66c PE; CEAL; NCA; CEACAM6; CEA cell adhesion molecule 6; anti-CD66c antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: B6.2
• Specificity: The mouse monoclonal antibody B6.2 recognizes a conformationally dependent epitope of native CD66c, a GPI-anchored extracellular glycoprotein expressed on granulocytes and epithelial cells.
• Purity/Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide

• Applicable Applications for anti-CD66c antibody: Flow Cytometry (FC/FACS)(QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests.
• Conjugation: PE
• Immunogen: Extracts from human breast carcinoma cells
• Excitation Laser: blue (488 nm)
• Workshop: HLDA V: MA135, HLDA VI: MA86
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD66c (B6.2) PE antibody (10ul reagent/100ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD66c (B6.2) PE antibody (10ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD66c antibody

CD66c is a GPI-anchored glycoprotein capable of homophilic adhesion and heterophilic binding to CD66a-e, CD62E, and galectins. It is expressed on granulocytes and epithelial cells, and has potential applications in the detection of sites of infection and inflammation.

References

Szpak CA, Johnston WW, Lottich SC, Kufe D, Thor A, Schlom J: Patterns of reactivity of four novel monoclonal antibodies (B72.3, DF3, B1.1 and B6.2) with cells in human malignant and benign effusions. Acta Cytol. 1984 Jul-Aug;28(4):356-67., URL: https://www.ncbi.nlm.nih.gov/pubmed/6205527
Yamagami S, Yokoo S, Amano S, Ebihara N: Characterization of bone marrow derived cells in the substantia propria of the human conjunctiva. Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4476-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/17898268
Watt SM, Teixeira AM, Zhou GQ, Doyonnas R, Zhang Y, Grunert F, Blumberg RS, Kuroki M, Skubitz KM, Bates PA: Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site. Blood. 2001 Sep 1;98(5):1469-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/11520797
Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K: Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow. J Biol Chem. 2008 Jun 6;283(23):15647-55., URL: https://pubmed.ncbi.nlm.nih.gov/18375392/

NCBI and Uniprot Product Information

• NCBI GI #: 612407822
• NCBI GeneID: 4680
• NCBI Accession #: NP_002474.4
• NCBI GenBank Nucleotide #: NM_002483.6
• UniProt Accession #: P40199; Q13774; Q14920; Q53XP7
• Molecular Weight: 37,195 Da
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 6 preproprotein
• NCBI Official Synonym Full Names: carcinoembryonic antigen related cell adhesion molecule 6
• NCBI Official Symbol: CEACAM6
• NCBI Official Synonym Symbols: NCA; CEAL; CD66c
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 6
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 6
• UniProt Gene Name: CEACAM6
• UniProt Synonym Gene Names: NCA

NCBI Description

This gene encodes a protein that belongs to the carcinoembryonic antigen (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. Members of this family play a role in cell adhesion and are widely used as tumor markers in serum immunoassay determinations of carcinoma. This gene affects the sensitivity of tumor cells to adenovirus infection. The protein encoded by this gene acts as a receptor for adherent-invasive E. coli adhesion to the surface of ileal epithelial cells in patients with Crohn's disease. This gene is clustered with genes and pseudogenes of the cell adhesion molecules subgroup of the CEA family on chromosome 19. [provided by RefSeq, Apr 2014]

Uniprot Description

CEACAM6: a glycosylphosphatidylinositol(GPI)-linked glycoprotein implicated in a variety of human cancers. Expressed on epithelial cells of various tissues, especially colon, lung, pancreas, trachea, and bone marrow. Participates in innate immune defense, cell proliferation and differentiation. Expressed at high levels in gastrointestinal tract, pancreatic and lung tumors. CEACAM6 promotes tumor angiogenesis in gastric cancer. May enhance invasiveness and metastatic potential in gastric and pancreatic cancer by promoting EMT via PI3K/AKT signaling. Upregulated by the pro-inflammatory cytokine IL-6. Belongs to the immunoglobulin superfamily/CEA family.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell-cell signaling; leukocyte migration; positive regulation of cell migration; positive regulation of cell proliferation; signal transduction