Mouse CD51/CD61 Antibody | anti-CD51/CD61 antibody

Anti-Hu CD51/CD61 PE

Cat. Num. AAA1802263

• Reactivity: Human, Chicken
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD51/CD61; Anti-Hu CD51/CD61 PE; VNRA; MSK8; ITGB3; ITGAV; Integrin alpha5beta3; OFA; VR; anti-CD51/CD61 antibody

• Host: Mouse
• Reactivity: Human, Chicken
• Isotype: Mouse IgG1
• Clone Number: 23C6
• Specificity: The mouse monoclonal antibody 23C6 recognizes an extracellular epitope on CD51/CD61 complex, that is expressed mainly on human osteoclasts, but also e.g. on placenta, or melanoma cell lines. The epitope is native and sensitive to fixation. In chicken this antibody can be used to selectively identify the thrombocytes.
• Purity/Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide

• Applicable Applications for anti-CD51/CD61 antibody: Flow Cytometry (FC/FACS)(QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests.
• Negative Species: Rabbit, Pig, Cow
• Conjugation: PE
• Immunogen: A cell suspension containing osteoclasts from osteoclastomas
• Excitation Laser: blue (488 nm)
• Workshop: HLDA IV
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(Separation of REH cells stained using anti-human CD51/CD61 (23C6) PE antibody (10ul reagent per million cells in 100ul of cell suspension, red-filled) from REH cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 7ug/ml, same as CD51/CD61 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of REH cell suspension.)

Related Product Information for anti-CD51/CD61 antibody

CD51/CD61 (integrin alpha5beta3), also known as osteoclast functional antigen, serves as a vitronectin receptor, and binds also to fibronectin, fibrinogen, thrombospondin, osteopontin, collagen, and von Willebrand factor. Expression of this antigen increases with melanoma progression. In healthy individuals CD51/CD61 is expressed mainly on osteoclasts, placenta, and endothelial cells, at lower levels on platelets and macrophages.

References

Viertlboeck BC, Göbel TW: Chicken thrombocytes express the CD51/CD61 integrin. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):137-41, URL: https://pubmed.ncbi.nlm.nih.gov/17659354/
Yamamoto T, Kurihara N, Yamaoka K, Ozono K, Okada M, Yamamoto K, Matsumoto S, Michigami T, Ono J, Okada S: Bone marrow-derived osteoclast-like cells from a patient with craniometaphyseal dysplasia lack expression of osteoclast-reactive vacuolar proton pump. J Clin Invest. 1993 Jan;91(1):362-7., URL: https://pubmed.ncbi.nlm.nih.gov/7678608/
Leukocyte Typing IV., Knapp WB. et al. (Eds.), Oxford University Press (1989).
Davies J, Warwick J, Totty N, Philp R, Helfrich M, Horton M: The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor. J Cell Biol. 1989 Oct;109(4 Pt 1):1817-26., URL: https://pubmed.ncbi.nlm.nih.gov/2477382/
Barua A, Bitterman P, Bahr JM, Bradaric MJ, Hales DB, Luborsky JL, Abramowicz JS: Detection of tumor-associated neoangiogenesis by Dopplerultrasonography during early-stage ovarian cancer in laying hens: a preclinical model of human spontaneous ovarian cancer. Jultrasound Med. 2010 Feb;29(2):173-82., URL: https://pubmed.ncbi.nlm.nih.gov/20103787/