Immunocytochemistry (ICC)
(Immunocytochemistry using MBS600591 at a 1:1000 dilution.)
Mouse Giantin Monoclonal Antibody | anti-GOLGB1 antibody
Giantin (Macrogolgin, Golgi Complex-associated 372kD Protein, GCP372)
Gene Names
GOLGB1; GCP; GCP372; GOLIM1
Reactivity
Canine, Goat, Human, Monkey, Mouse, Porcine, Rat, Sheep
Applications
ELISA, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Giantin; Monoclonal Antibody; Giantin (Macrogolgin; Golgi Complex-associated 372kD Protein; GCP372); Anti -Giantin (Macrogolgin; anti-GOLGB1 antibody
Host
Mouse
Reactivity
Canine, Goat, Human, Monkey, Mouse, Porcine, Rat, Sheep
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D410
Specificity
Recognizes monkey giantin. Species Crossreactivity: human, mouse, rat, goat, sheep, canine, porcine, mammalian and avian cells.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide.
Applicable Applications for anti-GOLGB1 antibody
ELISA (EL/EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Not recommended for Western Blot.,
Dilution: Immunocytochemistry: 1:1000
Immunoprecipitation: 2ul
Immunohistochemistry: Frozen sections
Optimal dilution to be determined by researcher.
Dilution: Immunocytochemistry: 1:1000
Immunoprecipitation: 2ul
Immunohistochemistry: Frozen sections
Optimal dilution to be determined by researcher.
Immunogen
Giantin from Caco-2 cell golgi membrane fraction.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunocytochemistry (ICC)
(Immunocytochemistry using MBS600591 at a 1:1000 dilution.)
Immunocytochemistry (ICC)
(Immunocytochemistry using MBS600591 at a 1:1000 dilution.)
Related Product Information for anti-GOLGB1 antibody
Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. Giantin plays also a role in docking COPI vesicles to Golgi membranes. It is speculated that after mitosis giantin aids in guiding vesiculated Golgi membranes to the microtubule organizing center to reform a new Golgi apparatus.
Product Categories/Family for anti-GOLGB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
376,019 Da
NCBI Official Full Name
Golgin subfamily B member 1 isoform 4
NCBI Official Synonym Full Names
golgin B1
NCBI Official Symbol
GOLGB1
NCBI Official Synonym Symbols
GCP; GCP372; GOLIM1
NCBI Protein Information
Golgin subfamily B member 1; giantin; macrogolgin; golgi integral membrane protein 1; 372 kDa Golgi complex-associated protein; golgin B1, golgi integral membrane protein; golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1
UniProt Protein Name
Golgin subfamily B member 1
Protein Family
UniProt Gene Name
GOLGB1
UniProt Synonym Gene Names
GCP372
UniProt Entry Name
GOGB1_HUMAN
Uniprot Description
GOLGB1: May participate in forming intercisternal cross-bridges of the Golgi complex.
Protein type: Vesicle; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q13
Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; cis-Golgi network; membrane; ER-Golgi intermediate compartment; integral to membrane
Molecular Function: protein binding
Biological Process: Golgi organization and biogenesis
Research Articles on GOLGB1
Similar Products
Product Notes
The GOLGB1 golgb1 (Catalog #AAA600591) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Giantin (Macrogolgin, Golgi Complex-associated 372kD Protein, GCP372) reacts with Canine, Goat, Human, Monkey, Mouse, Porcine, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Giantin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Not recommended for Western Blot., Dilution: Immunocytochemistry: 1:1000 Immunoprecipitation: 2ul Immunohistochemistry: Frozen sections Optimal dilution to be determined by researcher. Researchers should empirically determine the suitability of the GOLGB1 golgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Giantin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
