Testing Data
Mammalian Phenylalanine Ammonia-Lyase Assay Kit
Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric)
Reactivity
Mammalian
Synonyms
Phenylalanine Ammonia-Lyase; Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric); Phenylalanine Ammonia-Lyase assay kit
Reactivity
Mammalian
Specificity
Measurement of Phenylalanine ammonia-lyase activity in samples. Can detect as low as 3 uU (3 pmol substrate catalysis/minute) of PAL under assay conditions.
Detection Method
Fluorescence (Ex/Em = 410/470nM)
Sample Type
Purified protein or recombinant enzyme (must be free of primary amines including ammonium sulfate), Serum
Features & Benefits
The assay is simple. Sensitive. A facile, rapid way to measure Phenylalanine ammonia-lyase activity in samples.
Preparation and Storage
Store at -20 degree C
Shipping: Gel Pack
Shelf Life: Est.~12 months
Shipping: Gel Pack
Shelf Life: Est.~12 months
Testing Data
Testing Data
Related Product Information for Phenylalanine Ammonia-Lyase assay kit
Phenylalanine Ammonia-Lyase (PAL, EC 4.3.1.24) catalyzes the reversible non-oxidative deamination of L-phenylalanine to trans-cinnamic acid and ammonia without any external cofactors. PAL is expressed in plants, yeast and some fungi and is absent in bacteria and animals. Patients suffering from the genetic disorder, phenylketonuria is unable to metabolize phenylalanine that leads to irreversible cognitive impairment. Historically, phenylketonuria has been treated with a phenylalanine-restricted diet taken for the entirety of the patient's life. Recently, a drug containing PEGylated PAL has been FDA-approved to treat phenylketonuria, limiting the need of a restricted diet. Phenylalanine Ammonia-Lyase Activity Assay Kit measures the activity of PAL via a two-step plate-based assay. The products of the PAL enzymatic reaction, generated in the first step, react with the developers resulting in the formation of a fluorescent compound, which is detected at Ex/Em = 410/470nM. The kit can detect as low as 3 uUnits (3 pmol substrate catalysis per minute) of PAL under assay conditions.
Product Categories/Family for Phenylalanine Ammonia-Lyase assay kit
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Product Notes
The Mammalian Phenylalanine Ammonia-Lyase (Catalog #AAA8400635) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8400635 Assay Kit recognizes Mammalian Phenylalanine Ammonia-Lyase. It is sometimes possible for the material contained within the vial of "Phenylalanine Ammonia-Lyase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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