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Testing Data

Homocysteine Lyase Assay Kit

Homocysteine Lyase Activity Assay Kit (Fluorometric)

Reactivity
Bacterial samples
Synonyms
Homocysteine Lyase; Homocysteine Lyase Activity Assay Kit (Fluorometric); Homocysteine Lyase assay kit
Ordering
For Research Use Only!
Reactivity
Bacterial samples
Samples
Bacterial celllysate
Detection Method
Fluorescence: Ex/Em 368/460nm
Features & Benefits
Sensitive method to assay HCYase activity in bacterial samples
Quick
Simple
Determining the activity of wild type or recombinant Homocysteine alpha,gamma-Lyase
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months

Testing Data

Testing Data
Related Product Information for Homocysteine Lyase assay kit
Homocysteine alpha,gamma-Lyase (HCYase) is a pyridoxal 5'-phosphate dependent enzyme that catalyzes the breakdown of homocysteine to hydrogen sulfide, ammonia, and alpha-ketobutyrate. HCYase is present in high activity in the anaerobic protozoan parasite Trichomonas vaginalis but is absent from mammals. The Trichomonas HCYase is highly selective for homocysteine as compared to cysteine. Additionally, it has broad pH stability (pH 6-8) and is thermally stable up to 50 degree C. This makes rHCYase a promising candidate for therapeutic applications including the detection of hyperhomocystenemia, which has been demonstrated to be a major risk factor in cardiovascular diseases. Homocysteine Lyase Activity Assay Kit is a simple, quick and sensitive method to assay HCYase activity in bacterial samples. The assay relies on the HCYase-catalyzed release of hydrogen sulfide (H2S), which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm.

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Product Notes

The Bacterial samples Homocysteine Lyase (Catalog #AAA849600) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849600 Assay Kit recognizes Bacterial samples Homocysteine Lyase. It is sometimes possible for the material contained within the vial of "Homocysteine Lyase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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