Testing Data
General Sialyltransferase Assay Kit
Sialyltransferase Activity Assay Kit (Fluorometric)
Reactivity
General
Synonyms
Sialyltransferase; Sialyltransferase Activity Assay Kit (Fluorometric); Sialyltransferase assay kit
Reactivity
General
Specificity
Measurement of Sialyltransferase activity using a 96-well plate format. The kit can measure as low as 20 uU ST activity under assay conditions and can detect activity from both alpha-1; 6 and alpha-2; 6 sialyltransferases
Detection Method
Fluorescence (Ex/Em = 410/470nM)
Sample Type
Recombinant enzyme, Purified protein, Tissue or cell lysate
Features & Benefits
A simple. Two-step plate based assay that measures the fluorescent product at Ex/Em = 410/470nM, For measuring Sialyltransferase activity using a 96-well plate format. The kit can detect activity from both alpha-1, 6 and alpha-2, 6 sialyltransferases
Preparation and Storage
Store at -20 degree C
Shipping: Gel Pack
Shelf Life: Est.~12 months
Shipping: Gel Pack
Shelf Life: Est.~12 months
Testing Data
Testing Data
Related Product Information for Sialyltransferase assay kit
Sialyltransferases (ST, Galactosyldiacylglycerol alpha-2, 3-sialyltransferase; 2.4.99.5) belong to the glycosyltransferase family and are expressed in a variety of organisms from bacteria to higher mammals. Previously, it was believed to be not expressed in plants, but sialyltransferase like proteins have been recently identified in rice. STs catalyze the transfer of sialic acid (a kind of N-acetylneuraminic acid) from CMP-Neu5Ac moieties to sugar molecules on the terminal regions of glycolipids and glycoproteins. In higher animals, terminal sialic acid residue-containing glycoconjugates have various important functions including modulation of the immune system, brain development, etc. In bacteria, they play a role in virulence and are present on the bacterial surface to protect them from the immune system. Sialyltransferase Activity Assay Kit is a simple, two-step plate based assay that measures the fluorescent product at Ex/Em = 410/470nM. The assay consists of three enzymatic steps leading to the generation of ammonia. Ammonia then reacts with the detection reagent to form a fluorescent product measured at Ex/Em = 410/470nM. The kit can detect activity from both alpha-1, 6 and alpha-2, 6 sialyltransferases and can measure as low as 20 uU ST activity under assay conditions.
Product Categories/Family for Sialyltransferase assay kit
Similar Products
Product Notes
The General Sialyltransferase (Catalog #AAA8400630) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8400630 Assay Kit recognizes General Sialyltransferase. It is sometimes possible for the material contained within the vial of "Sialyltransferase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
