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Testing Data

General Phytase Assay Kit

Phytase Activity Assay Kit (Fluorometric)

Reactivity
General
Synonyms
Phytase; Phytase Activity Assay Kit (Fluorometric); Phytase assay kit
Ordering
For Research Use Only!
Reactivity
General
Specificity
Measurement of phytase activity in various samples. Can detect as low as 1 uU of phytase activity under assay conditions.
Detection Method
Fluorescence (Ex/Em = 535/587nM)
Sample Type
Grains: Barley seeds, Fungus: Mushrooms
Features & Benefits
A facile, rapid way to monitor phytase activity in various biological samples. The assay is simple. Sensitive.
Preparation and Storage
Store at -20 degree C
Shipping: Gel Pack
Shelf Life: Est.~12 months

Testing Data

Testing Data
Related Product Information for Phytase assay kit
Phytase belongs to the phosphatase enzyme family that hydrolyzes phytic acid, an indigestible form of phosphorous to inositol and free inorganic phosphorous. Phytic acid is often referred to as an anti-nutrient because it binds to minerals such as calcium, iron, zinc, potassium, magnesium in the digestive tract and reduces its absorption thereby leading to bone loss and toxic build-up. Phytase is produced by bacteria in the gut of ruminant animals such as cattle, sheep etc. making it possible for them to use the phytic acid found in grains, plant tissues as a source of phosphorus. However, animals such as dogs, pigs do not produce phytase and thus, their feed is supplemented with phytase. Phytase can reduce the anti-nutritional effect of phytate and improve the digestibility of phosphorous, calcium, amino acids, carbohydrates etc. Phytases can also be used as a human supplement to boost bone health and reduce osteoporosis. Phytase Activity Assay Kit provides a facile, rapid way to monitor phytase activity in various biological samples. In this kit, phytase cleaves the phytic acid substrate and releases phosphate, which is then used to release glucose from a disaccharide. In subsequent reactions, glucose reacts with the probe and the developer to generate a fluorescent product measured at Ex/Em = 535/587nM. The assay is simple, sensitive, and can detect as low as 1 uU of phytase activity under assay conditions.

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Product Notes

The General Phytase (Catalog #AAA8400634) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8400634 Assay Kit recognizes General Phytase. It is sometimes possible for the material contained within the vial of "Phytase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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