Typical Testing Data/Standard Curve (for reference only)
General PicoProbe Fructose-1; 6-Bisphosphate Assay Kit
PicoProbe Fructose-1; 6-Bisphosphate Assay Kit (F)
Reactivity
General
Synonyms
PicoProbe Fructose-1; 6-Bisphosphate; 6-Bisphosphate Assay Kit (F); 6-Bisphosphate assay kit
Reactivity
General
Specificity
Measurement of F1; 6BP in various tissues or cell lysates; Analysis of Glycolysis Pathway and Pentose Phosphate Pathway
Detection Method
Fluorescence (Ex/Em = 535/587nM)
Sample Type
Plant tissues: Germinated Barley Seed, Spinach etc., Animal tissues: Liver, Kidney etc.
Features & Benefits
Simple. A quick and easy way to measure Fructose-1, 6-Bisphosphate in a variety of sample types. Sensitive. Easy to perform
Preparation and Storage
Store at -20 degree C
Shipping: Gel Pack
Shelf Life: Est.~12 months
Shipping: Gel Pack
Shelf Life: Est.~12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PicoProbe Fructose-1; 6-Bisphosphate assay kit
Fructose-1, 6-Bisphosphate (F1, 6BP) is a fructose sugar and a key component of the glycolysis metabolic pathway. It is produced by the phosphorylation of Fructose 6-Phosphate (F6P) by phosphofructokinase (PFK). Additionally, F1, 6BP is an allosteric activator of enzymes including PFK and pyruvate kinase. Furthermore, F1, 6BP stimulates the Pentose Phosphate Pathway that produces glutathione and is associated with anti-convulsing effects in animal models. Recent studies have shown that F1, 6BP can be taken up by various cell types thereby providing ATP in glycolysis without consuming ATP and preserving the organs such as heart, liver, kidney under ischemic conditions. PicoProbe Fructose-1, 6-Bisphosphate Assay Kit provides a quick and easy way to measure F1, 6BP in a variety of sample types. In this Assay, F1, 6BP is hydrolyzed by Fructose-1, 6-Bisphosphatase (FBP) into inorganic phosphate and F6P. F6P then through several intermediate reactions reacts with the PicoProbe to generate a stable fluorophore measured at Ex/Em = 535/587nM. The assay is simple, easy to perform, sensitive and can detect F1, 6BP less than 0.5uM in a variety of samples.
Product Categories/Family for PicoProbe Fructose-1; 6-Bisphosphate assay kit
Similar Products
Product Notes
The General PicoProbe Fructose-1; 6-Bisphosphate (Catalog #AAA8400619) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8400619 Assay Kit recognizes General PicoProbe Fructose-1; 6-Bisphosphate. It is sometimes possible for the material contained within the vial of "PicoProbe Fructose-1; 6-Bisphosphate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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