Testing Data
Eukaryotes Cytidine Deaminase Assay Kit
Cytidine Deaminase Activity Assay Kit (Fluorometric)
Reactivity
Eukaryotes
Synonyms
Cytidine Deaminase; Cytidine Deaminase Activity Assay Kit (Fluorometric); CDA Activity Assay Kit (Fluorometric); Cytidine Deaminase assay kit
Reactivity
Eukaryotes
Samples
Animal tissues: mouse or rat spleen, liver, and kidney
Detection Method
Fluorescence: Ex/Em 410/470nm
Features & Benefits
Rapid & simple assay to measures total Cytidine deaminase activity
The kit measures total Activity of Cytidine Deaminase with limit of quantification 1 uU recombinant Cytidine Deaminase
Range of the assay: 10-100 uU.
Measurement of CDA activity in various tissues/cellsEvaluation of CDA activity for comparison of activity rates in normal versus tumor tissues
Rapid & simple assay to measures total Cytidine deaminase activity
The kit measures total Activity of Cytidine Deaminase with limit of quantification 1 uU recombinant Cytidine Deaminase
Range of the assay: 10-100 uU.
Measurement of CDA activity in various tissues/cellsEvaluation of CDA activity for comparison of activity rates in normal versus tumor tissues
Rapid & simple assay to measures total Cytidine deaminase activity
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Cytidine Deaminase assay kit
Cytidine Deaminase (3.5.4.5) is a homotetramer enzyme that catalyzes the irreversible deamination of cytidine to produce uridine, thus maintaining the intracellular pyrimidine pool of uridine. Cytidine as well as adenosine, thymidine, guanosine and uridine are one of the five standard nucleosides with which nucleic acids are composed. Nucleosides accumulate or are recycled from salvage pathways within the cell and play an important role in DNA and RNA synthesis and subsequent cell division. Tumorigenesis is intricately linked to metabolism of macromolecular precursors, specifically, the building blocks of DNA and RNA. Thus tumor cells have greater concentrations of uridine and cytidine analogues (402 uM +/- 252) compared to normal cells (83 uM +/- 133), to support the increased demand for nucleotides in mitosis in actively dividing cells. Cytidine Deaminase Activity Assay Kit uses CDA to convert cytidine to the uridine and NH3, as intermediates. The intermediate products then react with a proprietary Reaction Mix to generate a stable fluorophore that can be detected fluorometrically (Ex/Em= 410/470 nm). The kit is suitable for measuring CDA activity as low as 1 uUnit (1 pmole/min/well) in biological samples
Product Categories/Family for Cytidine Deaminase assay kit
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Product Notes
The Eukaryotes Cytidine Deaminase (Catalog #AAA849673) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849673 Assay Kit recognizes Eukaryotes Cytidine Deaminase. It is sometimes possible for the material contained within the vial of "Cytidine Deaminase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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