PicoProbe NADPH Quantitation Assay Kit
PicoProbe NADPH Quantitation Fluorometric Assay Kit
Analysis of metabolism and cell signaling in various cells
Cell culture: Adherent or suspension cells
Mitochondria
The kit can measure less than 40 nM NADPH in a variety of samples
Shelf Life: 12 months
Background: NADP (nicotinamide dinucleotide) is a major electron donor for anabolic reactions such as lipid and nucleic acid synthesis. The reduced form, NADPH is a cofactor for many redox enzymes. It is of increasing interest to be able to measure low level of intracellular NADPH. MyBioSource's PicoProbe NADPH Quantitation Assay kit provides a highly sensitive method to detect intracellular NADPH. NADH is not detected as the NADPH Recycling Enzyme Mix specifically recognizes NADPH in the enzyme recycling reaction. The assay is rapid, simple and does not require purifying NADPH from samples. The kit can measure less than 40 nM NADPH in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm)
Applications: - Measurement of NADPH in various tissues/cells - Analysis of metabolism and cell signaling in various cells
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Product Notes
The PicoProbe NADPH Quantitation (Catalog #AAA841904) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of NADPH in various tissues/cells Analysis of metabolism and cell signaling in various cells. Researchers should empirically determine the suitability of the PicoProbe NADPH Quantitation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PicoProbe NADPH Quantitation, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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