Typical Testing Data/Standard Curve (for reference only)
PicoProbe Glucose-6-Phosphate Dehydrogenase Assay Kit
PicoProbe Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)
Synonyms
PicoProbe Glucose-6-Phosphate Dehydrogenase; PicoProbe Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric); PicoProbe G6PDH Activity Assay Kit (Fluorometric); PicoProbe Glucose-6-Phosphate Dehydrogenase assay kit
Application Notes
Measurement of G6PDH activity in various tissues and cells
Evaluation of pentose phosphate pathway
Evaluation of pentose phosphate pathway
Samples
Animal tissues: muscle, liver, heart, kidney, etc.
Cell culture: adherent or suspension cells
Plant tissues
Cell culture: adherent or suspension cells
Plant tissues
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Features & Benefits
Rapid & simple
High-throughput adaptable
Can detect as low as 1 uU of G6PDH activity
High-throughput adaptable
Can detect as low as 1 uU of G6PDH activity
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PicoProbe Glucose-6-Phosphate Dehydrogenase assay kit
Product Information: PicoProbe Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit: The most sensitive assay for measuring G6PDH activity in a variety of samples. Detection Limit: as low as 1 uU. 100 Assays
Background: Glucose-6-Phosphate Dehydrogenase (G6PDH: EC 1.1.1.49) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as liver, mammary gland, adipose tissue, and adrenal gland. MyBioSource's PicoProbe Glucose-6-Phosphate Dehydrogenase Assay kit provides a quick and easy method for monitoring G6PDH activity in a wide variety of samples. In this assay, G6PDH converts G6P into pyruvate and NADPH, which further reduces PicoProbe to generate an intense fluorescence product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 1 uU of G6PDH activity.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm)
Application- Measurement of G6PDH activity in various tissues and cells - Evaluation of pentose phosphate pathway
Background: Glucose-6-Phosphate Dehydrogenase (G6PDH: EC 1.1.1.49) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as liver, mammary gland, adipose tissue, and adrenal gland. MyBioSource's PicoProbe Glucose-6-Phosphate Dehydrogenase Assay kit provides a quick and easy method for monitoring G6PDH activity in a wide variety of samples. In this assay, G6PDH converts G6P into pyruvate and NADPH, which further reduces PicoProbe to generate an intense fluorescence product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 1 uU of G6PDH activity.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm)
Application- Measurement of G6PDH activity in various tissues and cells - Evaluation of pentose phosphate pathway
Product Categories/Family for PicoProbe Glucose-6-Phosphate Dehydrogenase assay kit
Similar Products
Product Notes
The PicoProbe Glucose-6-Phosphate Dehydrogenase (Catalog #AAA841758) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of G6PDH activity in various tissues and cells Evaluation of pentose phosphate pathway. Researchers should empirically determine the suitability of the PicoProbe Glucose-6-Phosphate Dehydrogenase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PicoProbe Glucose-6-Phosphate Dehydrogenase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
