Typical Testing Data/Standard Curve (for reference only)
Adenosylhomocysteinase (AHCY) Assay Kit | Ahcy assay kit
Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit
Cell and tissue lysate
The assay can detect AHCY activity as low as 1 uU recombinant human AHCY.
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington's disease, while in Wilson's disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease. MyBioSource's AHCY Activity Assay kit can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the OxiRed Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification (L.O.Q) is 1 uU recombinant human AHCY.
Summary: Detection Method - Fluorescence (Ex/Em = 535/587 nm)
Application: Detection of AHCY activity
NCBI and Uniprot Product Information
NCBI Description
enzyme that catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine [RGD, Feb 2006]
Uniprot Description
SAHH: Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD). A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. Belongs to the adenosylhomocysteinase family.
Protein type: Other Amino Acids Metabolism - selenoamino acid; EC 3.3.1.1; Hydrolase; Amino Acid Metabolism - cysteine and methionine
Cellular Component: neuron projection; cytoplasm; melanosome; cytosol; nucleus
Molecular Function: identical protein binding; adenyl nucleotide binding; copper ion binding; protein self-association; adenosylhomocysteinase activity; NAD binding
Biological Process: circadian sleep/wake cycle; S-adenosylmethionine cycle; response to hypoxia; S-adenosylhomocysteine catabolic process; one-carbon compound metabolic process; chronic inflammatory response to antigenic stimulus; response to nutrient
Research Articles on Ahcy
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Product Notes
The Ahcy ahcy (Catalog #AAA841507) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Detection of AHCY activity. Researchers should empirically determine the suitability of the Ahcy ahcy for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenosylhomocysteinase (AHCY), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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