Typical Testing Data/Standard Curve (for reference only)
Lipolysis (Adipocyte) Assay Kit
Lipolysis (Adipocyte) Colorimetric / Fluorometric Assay Kit
Synonyms
Lipolysis (Adipocyte); Lipolysis (Adipocyte) Colorimetric / Fluorometric Assay Kit; Lipolysis (Adipocyte) assay kit
Application Notes
Isolation of adipocytes from rat/mouse tissue
Measurement of lipolysis by adipocytes
Screening compounds that influence lipolysis, mechanistic studies and studies on metabolic dysfunction
Measurement of lipolysis by adipocytes
Screening compounds that influence lipolysis, mechanistic studies and studies on metabolic dysfunction
Samples
Animal tissues: adipocytes
Detection Method
Absorbance (OD 570 nm) and Fluorescence (Ex/Em = 535/587 nm)
Features & Benefits
Simple, rapid & convenient method to isolate adipocytes from adipose tissue & measure lipolysis.
The assay kit can detect less than 200 pmol glycerol in the colorimetric assay and less than 20 pmol in the fluorometric assay.
The assay kit can detect less than 200 pmol glycerol in the colorimetric assay and less than 20 pmol in the fluorometric assay.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Lipolysis (Adipocyte) assay kit
Product Information: Lipolysis (Adipocyte) Colorimetric/Fluorometric Assay Kit: Only kit on the market to isolate adipocytes from adipose tissue & measure lipolysis. Detection limit: less than 20 pmol. For 5 g tissue.
Background: Lipolysis is the intracellular hydrolysis of triglycerides into glycerol and free fatty acids, which are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by hormones or catecholamine, which binds to beta-adrenergic receptors leading to activation of adenylate cyclase, forming cAMP from ATP. cAMP serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides. This pathway is inhibited by insulin. MyBioSource's Adipocyte Lipolysis Colorimetric/Fluorometric Assay kit is a simple, facile way to monitor lipolysis. The kit provides all reagents necessary to isolate adipocytes from up to 5 g of mouse or rat adipose tissue and measure glycerol release from primary adipocytes after induction of lipolysis. It also includes the synthetic catecholamine, Isoproterenol, to stimulate the cAMP-mediated pathway. The signal intensity is directly proportional to the amount of glycerol present. This assay kit can detect less than 200 pmol glycerol in the colorimetric assay and less than 20 pmol in the fluorometric assay.
Summary: Detection method- Absorbance (OD 570 nm) and Fluorescence (Ex/Em = 535/587 nm).
Applications-
Isolation of adipocytes from rat/mouse tissue
Measurement of lipolysis by adipocytes
Screening compounds that influence lipolysis, mechanistic studies and studies on metabolic dysfunction
Background: Lipolysis is the intracellular hydrolysis of triglycerides into glycerol and free fatty acids, which are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by hormones or catecholamine, which binds to beta-adrenergic receptors leading to activation of adenylate cyclase, forming cAMP from ATP. cAMP serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides. This pathway is inhibited by insulin. MyBioSource's Adipocyte Lipolysis Colorimetric/Fluorometric Assay kit is a simple, facile way to monitor lipolysis. The kit provides all reagents necessary to isolate adipocytes from up to 5 g of mouse or rat adipose tissue and measure glycerol release from primary adipocytes after induction of lipolysis. It also includes the synthetic catecholamine, Isoproterenol, to stimulate the cAMP-mediated pathway. The signal intensity is directly proportional to the amount of glycerol present. This assay kit can detect less than 200 pmol glycerol in the colorimetric assay and less than 20 pmol in the fluorometric assay.
Summary: Detection method- Absorbance (OD 570 nm) and Fluorescence (Ex/Em = 535/587 nm).
Applications-
Isolation of adipocytes from rat/mouse tissue
Measurement of lipolysis by adipocytes
Screening compounds that influence lipolysis, mechanistic studies and studies on metabolic dysfunction
Product Categories/Family for Lipolysis (Adipocyte) assay kit
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Product Notes
The Lipolysis (Adipocyte) (Catalog #AAA841676) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Isolation of adipocytes from rat/mouse tissue Measurement of lipolysis by adipocytes Screening compounds that influence lipolysis, mechanistic studies and studies on metabolic dysfunction. Researchers should empirically determine the suitability of the Lipolysis (Adipocyte) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lipolysis (Adipocyte), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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