Typical Testing Data/Standard Curve (for reference only)
Sphingomyelinase Assay Kit
Sphingomyelinase Activity Fluorometric Assay Kit
Synonyms
Sphingomyelinase; Sphingomyelinase Activity Fluorometric Assay Kit; Sphingomyelinase assay kit
Application Notes
Measurement of SMase activity in various tissues/cells extracts
Samples
Animal tissues: brain, heart, kidney, etc.
Cell culture: Adherent or suspension cells
Cell culture: Adherent or suspension cells
Detection Method
Fluorescence (Ex/Em 535/587 nm)
Features & Benefits
Simple, rapid & convenient
The assay can detect sphingomyleinase activity as low as 10 uU/ml in a variety of samples.
The assay can detect sphingomyleinase activity as low as 10 uU/ml in a variety of samples.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Sphingomyelinase assay kit
Product Information: Sphingomyelinase Activity Fluorometric Assay Kit: Simple & convenient assay to measure sphingomyleinase activity in tissue samples such as brain, heart etc. & adhesion or suspension cells. Detection limit: < 10 uU/ml. 100 assay
Background: Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation and sterol homeostasis. MyBioSource's SMase Fluorometric Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using fluorescence (Ex/Em = 535/587 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce fluorescence. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 10 uU/ml in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em 535/587 nm)
Applications- Measurement of SMase activity in various tissues/cells extracts
Background: Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation and sterol homeostasis. MyBioSource's SMase Fluorometric Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using fluorescence (Ex/Em = 535/587 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce fluorescence. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 10 uU/ml in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em 535/587 nm)
Applications- Measurement of SMase activity in various tissues/cells extracts
Product Categories/Family for Sphingomyelinase assay kit
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Product Notes
The Sphingomyelinase (Catalog #AAA841694) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of SMase activity in various tissues/cells extracts. Researchers should empirically determine the suitability of the Sphingomyelinase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingomyelinase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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