Typical Testing Data/Standard Curve (for reference only)
PicoProbe Lipolysis (3T3-L1) Assay Kit
PicoProbe Lipolysis (3T3-L1) Fluorometric Assay Kit
Synonyms
PicoProbe Lipolysis (3T3-L1); PicoProbe Lipolysis (3T3-L1) Fluorometric Assay Kit; PicoProbe Lipolysis (3T3-L1) assay kit
Application Notes
Measurement of lipolysis in 3T3-L1 cells or adipocytes
Screening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions
Screening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions
Samples
Primary adipocytes
Cell culture: 3T3-L1 cells
Cell culture: 3T3-L1 cells
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Features & Benefits
Simple, rapid & convenient
The assay can detect less than 20 pmol of glycerol.
The assay can detect less than 20 pmol of glycerol.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PicoProbe Lipolysis (3T3-L1) assay kit
Product Information: Lipolysis (3T3-L1) Fluorometric Assay Kit: The most sensitive assay to measure lipolysis in primary adipocytes & 3T3-L1 cells. Detection limit: < 20 pmol. 100 assay
Background: Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by catecholamines and certain hormones. The kit includes the synthetic catecholamine, Isoproterenol, which activates beta-adrenergic receptors. This leads to activation of adenylate cyclase, which catalyzes the conversion of ATP to cAMP. cAMP then serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides. This pathway can be inhibited by insulin. MyBioSource's Lipolysis (3T3-L1) Fluorometric Assay kit is simple, easy-to-use & the most sensitive kit on the market. This assay measures glycerol released from 3T3-L1 cells as early as 1 hr. after induction of lipolysis. It is suitable for measuring trace amounts of glycerol from samples. In this assay, glycerol reacts with Enzyme Mix to form an intermediate that in turn reacts with Glycerol Developer & PicoProbe to generate the fluorescence product. The fluorescence intensity is directly proportional to the amount of glycerol. This assay kit can detect less than 20 pmol of Glycerol.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm).
Applications- - Measurement of lipolysis in 3T3-L1 cells or adipocytes - Screening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions
Background: Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by catecholamines and certain hormones. The kit includes the synthetic catecholamine, Isoproterenol, which activates beta-adrenergic receptors. This leads to activation of adenylate cyclase, which catalyzes the conversion of ATP to cAMP. cAMP then serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides. This pathway can be inhibited by insulin. MyBioSource's Lipolysis (3T3-L1) Fluorometric Assay kit is simple, easy-to-use & the most sensitive kit on the market. This assay measures glycerol released from 3T3-L1 cells as early as 1 hr. after induction of lipolysis. It is suitable for measuring trace amounts of glycerol from samples. In this assay, glycerol reacts with Enzyme Mix to form an intermediate that in turn reacts with Glycerol Developer & PicoProbe to generate the fluorescence product. The fluorescence intensity is directly proportional to the amount of glycerol. This assay kit can detect less than 20 pmol of Glycerol.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm).
Applications- - Measurement of lipolysis in 3T3-L1 cells or adipocytes - Screening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions
Product Categories/Family for PicoProbe Lipolysis (3T3-L1) assay kit
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Product Notes
The PicoProbe Lipolysis (3T3-L1) (Catalog #AAA841808) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of lipolysis in 3T3-L1 cells or adipocytes Screening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions. Researchers should empirically determine the suitability of the PicoProbe Lipolysis (3T3-L1) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PicoProbe Lipolysis (3T3-L1), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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