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Testing Data

Eukaryotes D-Amino Acid Oxidase Assay Kit

D-Amino Acid Oxidase Activity Assay Kit (Fluorometric)

Reactivity
Eukaryotes
Synonyms
D-Amino Acid Oxidase; D-Amino Acid Oxidase Activity Assay Kit (Fluorometric); DAO; DAAO; D-Amino Acid Oxidase assay kit
Ordering
For Research Use Only!
Reactivity
Eukaryotes
Samples
Animal tissues: kidney, cerebellum,etc
Detection Method
Fluorescence: Ex/Em 535/587nm
Features & Benefits
The first kit in the market to measure active D-Amino Acid Oxidase in biological samples
Rapid & Convenient
The assay can detect as little as 0.5 uU of DAAO enzymatic activity.
Includes active DAAO as positive control
Measurement of DAAO activity in various biological samples
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months

Testing Data

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Testing Data
Related Product Information for D-Amino Acid Oxidase assay kit
D-amino acid oxidase (DAAO, DAO, EC 1.4.3.3) is a flavoprotein which oxidizes a D-amino acid forming hydrogen peroxide and the respective imino acid which in a second catalytic step by he same enzyme is hydrolyzed to a alpha-keto acid and ammonia. In recent years, DAAO has been studied for its possible role in schizophrenia. The simultaneous expression of DAAO and a putative product from G72 gene may potentially increase the risk of schizophrenia in patients. For example, increased DAAO expression and enzymatic activity have been reported in post mortem brain tissue samples from patients with schizophrenia compared to healthy controls. DAAO expression in human brain is very specific: it can only be found in the cerebellum and is not detectable in any other parts of the brain. DAAO regulates the physiological levels of D-amino acids such as D-serine, D-aspartate and D-alanine. D-serine acts as a modulator of N-Methyl-D-aspartate receptor (NMDA receptor, NMDAR), while D-aspartate and D-alanine are found to be elevated in both white matter and gray matter in patients' brains suffering from Alzheimer disease. D-amino Acid Oxidase Activity Assay Kit provides a quick and easy method for monitoring DAAO activities in a wide variety of samples. In this assay, DAAO converts D-amino acid into hydrogen peroxide as one byproduct. Consequently, the formed byproduct will react with a probe producing a strong fluorescent signal (Ex/Em= 535/587nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.5 uU of DAAO activity
Product Categories/Family for D-Amino Acid Oxidase assay kit

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Product Notes

The Eukaryotes D-Amino Acid Oxidase (Catalog #AAA849731) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849731 Assay Kit recognizes Eukaryotes D-Amino Acid Oxidase. It is sometimes possible for the material contained within the vial of "D-Amino Acid Oxidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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