Testing Data
Fructose-1,6-Bisphosphatase Assay Kit
Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric)
Synonyms
Fructose-1; 6-Bisphosphatase; 6-Bisphosphatase Activity Assay Kit (Colorimetric); 6-Bisphosphatase Activity; 6-Bisphosphatase assay kit
Samples
Animal tissues: Liver, Kidney etc
Cell culture:HeLa, Jurkat,etc
Cell culture:HeLa, Jurkat,etc
Detection Method
Colorimetric: OD: 450nm
Features & Benefits
Sensitive, specific assay
Validated with animal and cell culture lysates
Includes FBP positive control
First assay in the market that measure FBP enzymatic activity in biological assays
Analysis of gluconeogenesis pathway
Measurement of Fructose-1,6-Bisphosphatase Activity in various tissues/cells
Mechanistic studies of Molecular and/or Cell biology processes (diabetes, gluconeogenesis)
Validated with animal and cell culture lysates
Includes FBP positive control
First assay in the market that measure FBP enzymatic activity in biological assays
Analysis of gluconeogenesis pathway
Measurement of Fructose-1,6-Bisphosphatase Activity in various tissues/cells
Mechanistic studies of Molecular and/or Cell biology processes (diabetes, gluconeogenesis)
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Related Product Information for Fructose-1,6-Bisphosphatase assay kit
Fructose-1,6-Bisphosphatase (FBP) (EC 3.1.3.11) is a rate-limiting enzyme for gluconeogenesis. It hydrolyzes Fructose-1,6-Bisphosphate releasing inorganic phosphate and Fructose-6-Phosphate that is further used in the synthesis of glucose. In humans, Fructose-1,6-Bisphosphatase can be found in the form of two isozymes: FBP1 and FBP2. FBP1 is mainly expressed in liver and kidneys, while FBP2 is expressed in all tissues. Deficiency of Fructose-1,6-Bisphoshatase causes hypoglycemia, metabolic acidosis, and unexpected death in infants. Recent studies have found elevated FBP levels subjects dealing with obesity and diabetes. Furthermore, inhibition of FBP increases glucose utilization and insulin sensitivity. Therefore, FBP could serve as a potential target for treating type II diabetes (insulin resistance). FBP activity assay kit provides a quick, reliable test that allows the measurement of FBP enzymatic activity in various samples. In this Assay, FBP hydrolyzes Fructose-1,6-Bisphosphate into Fructose-6-Phosphate (F6P). F6P is used in an enzyme-coupled reaction which reduces a chromophore forming a product with a stable signal that can measured at OD = 450 nm. The assay is simple, sensitive and can detect lower than 100 uU of FBP in variety of samples.
Product Categories/Family for Fructose-1,6-Bisphosphatase assay kit
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Product Notes
The Fructose-1,6-Bisphosphatase (Catalog #AAA849740) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fructose-1,6-Bisphosphatase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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