Testing Data
Mammalian Monoacylglycerol Lipase (MAGL) Assay Kit
Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)
Reactivity
Mammalian
Synonyms
Monoacylglycerol Lipase (MAGL); Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric); Monoacylglycerol Lipase (MAGL) assay kit
Reactivity
Mammalian
Samples
Animal tissues: brain, intestine, etc
Cell culture: Adherent or suspension cells
Cell culture: Adherent or suspension cells
Detection Method
Fluorometric: Ex/Em 360/460nm
Features & Benefits
Industry's most sensitive kit to measure and quantitate MAGL enzymatic activity in biological samples
Simple one-step reaction
Easy Sample preparation; assay only 1-2 hrs
Non-radiometric fluorescent detection
HTP adaptable
Measurement of monoacylglycerol lipase activity in various tissues/cells
Simple one-step reaction
Easy Sample preparation; assay only 1-2 hrs
Non-radiometric fluorescent detection
HTP adaptable
Measurement of monoacylglycerol lipase activity in various tissues/cells
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Related Product Information for Monoacylglycerol Lipase (MAGL) assay kit
Monoacylglycerol Lipase (MAGL, E.C. 3.1.1.23) is an important enzyme for regulation of endocannabinoid signaling in human physiology. MAGL is a serine hydrolase that generates free fatty acid and glycerol from monoacylglycerols such as 2-arachidonoyl glycerol (2-AG), one of the principal endocannabinoids active in the nervous system. One free fatty acid that is released by MAGL activity, arachidonic acid, is a precursor for eicosanoids, a family of pro-inflammatory signaling molecules. The leftover glycerol moiety can also serve various purposes as a metabolic building block and energy source. Inhibition or inactivation of MAGL leads to 2-AG buildup, and this has myriad biological consequences. Antinociceptive effects, anti-inflammatory and neuroprotective effects in Parkinson's and Alzheimer's diseases as well as reduced addiction withdrawal symptoms are some of the outcomes of MAGL inhibition. In addition, the downstream eicosanoid signaling pathway is affected; this pathway plays a role in diverse physiological processes including inflammation, immunity, nociception and blood pressure. As a central node in the regulation of both lipid signaling and energy mobilization, MAGL is of considerable interest as a therapeutic target. Monoacylglycerol Lipase activity assay kit provides a quick, sensitive and easy way for measuring MAGL activity in various samples. In this assay, a fluorescent substrate is cleaved to generate arachidonic acid and fluorescent metabolite and the increased fluorescence is measured at Ex/Em 360/460 nm. To identify the signal generated specifically by MAGL, a specific inhibitor is included that allows the user to differentiate MAGL activity from other sources of fluorescence. The assay is simple to perform, high-throughput adaptable and can detect as low as 0.1 mU of MAGL activity
Product Categories/Family for Monoacylglycerol Lipase (MAGL) assay kit
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Product Notes
The Mammalian Monoacylglycerol Lipase (MAGL) (Catalog #AAA849733) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849733 Assay Kit recognizes Mammalian Monoacylglycerol Lipase (MAGL). It is sometimes possible for the material contained within the vial of "Monoacylglycerol Lipase (MAGL), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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