Testing Data
General Phosphoglycerate Kinase Assay Kit
Phosphoglycerate Kinase Activity Assay Kit (Colorimetric)
Reactivity
General
Synonyms
Phosphoglycerate Kinase; Phosphoglycerate Kinase Activity Assay Kit (Colorimetric); PGK1; Phosphoglycerate Kinase assay kit
Reactivity
General
Samples
Animal tissues: Muscle, Liver, etc
Cell culture: HeLa, Jurkat, etc
Cell culture: HeLa, Jurkat, etc
Detection Method
Colorimetric: OD 340nm
Features & Benefits
First kit in the market to measure PGK activity in biological samples
Sensitive (~ 50 mU),Fast & Convenient,Protocol takes ~60 minute to be completed,Simple procedure
Mechanistic study for cancer development: e.g. Gastric Cancer,Measurement of Phosphoglycerate Kinase (PGK)Activity in various tissues/cells.,Analysis of glycolytic Pathway and Gluconeogenesis Pathway
Sensitive (~ 50 mU),Fast & Convenient,Protocol takes ~60 minute to be completed,Simple procedure
Mechanistic study for cancer development: e.g. Gastric Cancer,Measurement of Phosphoglycerate Kinase (PGK)Activity in various tissues/cells.,Analysis of glycolytic Pathway and Gluconeogenesis Pathway
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Related Product Information for Phosphoglycerate Kinase assay kit
Phosphoglycerate Kinase (PGK1) (EC 2.7.2.3) participates in two of the most important biochemical pathways in living organisms: Glycolysis and Gluconeogenesis. PGK is responsible for the catalysis of the reversible reaction of 1,3-Bisphosphoglycerate (1,3-BPG) and ADP to 3-Phosphoglycerate (3-PG) and ATP. In humans, PGK1 and PGK2, two PGK isozymes, have been characterized. While PGK1 is X-Chromosome-linked variant and expressed in all cells, PGK2 is autosome-linked variant and uniquely expressed in spermatogenic cells. Recent studies have shown deficiency of Phosphoglycerate Kinase could lead to chronic hemolytic anemia, mental disorders and myopathy in humans, whereas PGK overexpression promotes gastric cancer cell invasiveness. Therefore, accurate measurement of Phosphoglycerate Kinase Activity is useful for mechanistic and possible test studies. PGK Activity Assay kit provides a quick and easy way for monitoring PGK activity in various samples. In the first step of this enzymatic assay, PGK converts 3-Phosphoglycerate and ATP to 1,3-Bisphosphoglycerate and ADP. The nascent intermediate is detected via a series of enzymatic reactions that lead the oxidation of NADH to NAD+, which can be easily detected.
Product Categories/Family for Phosphoglycerate Kinase assay kit
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Product Notes
The General Phosphoglycerate Kinase (Catalog #AAA849573) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849573 Assay Kit recognizes General Phosphoglycerate Kinase. It is sometimes possible for the material contained within the vial of "Phosphoglycerate Kinase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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