Testing Data
Eukaryotes Sorbitol Dehydrogenase Assay Kit
Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric)
Reactivity
Eukaryotes
Synonyms
Sorbitol Dehydrogenase; Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric); Sorbitol Dehydrogenase assay kit
Reactivity
Eukaryotes
Samples
Animal tissues: Liver, Kidney, etc
Cell culture:HeLa, Jurkat etc
Cell culture:HeLa, Jurkat etc
Detection Method
Absorbance: 450nm
Features & Benefits
Simple, rapid & convenient
Can measure SDH activity as low as 50 uU in a variety of samples
Analysis of polyol-pathway.
Studies of chemically-induced hepatotoxicity and acute liver injury.
Freshly obtained serum from animals with suspected liver damage
Measurement of Sorbitol Dehydrogenase Activity in various tissues/cells
Can measure SDH activity as low as 50 uU in a variety of samples
Analysis of polyol-pathway.
Studies of chemically-induced hepatotoxicity and acute liver injury.
Freshly obtained serum from animals with suspected liver damage
Measurement of Sorbitol Dehydrogenase Activity in various tissues/cells
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Testing Data
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Testing Data
Related Product Information for Sorbitol Dehydrogenase assay kit
Sorbitol Dehydrogenase (SDH), also referred to as L-iditol dehydrogenase (EC 1.1.1.14) participates in the polyol pathway. Also known as the sorbitol-aldose pathway, this pathway is divided into two steps: first, glucose is reduced to sorbitol via the catalytic action of aldose reductase (AR), while the second step is catalyzed by sorbitol dehydrogenase, which utilizes sorbitol as substrate and subsequently produces fructose. Sorbitol is not a cell membrane permeable molecule; whereas fructose can be further metabolized inside the cells. Recent studies found that deficiency of Sorbitol Dehydrogenase causes the intracellular accumulation of sorbitol which leads complications in diabetes. The enzyme is predominately expressed in the liver cells and is only released into the bloodstream following acute liver damage. Therefore, the presence of significant SDH activity in serum is a potential indicator of liver injury or disease. SDH assay kit provides a quick and easy way for monitoring SDH activity in various samples. In this Assay, Sorbitol Dehydrogenase utilizes a provided substrate while reducing NAD+ to form NADH. NADH reacts with the developer, leading to the formation of a chromophore with strong absorbance at OD 450 nm. The assay is simple, sensitive and can detect Sorbitol Dehydrogenase Activity less than 50 uU in variety of samples
Product Categories/Family for Sorbitol Dehydrogenase assay kit
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Product Notes
The Eukaryotes Sorbitol Dehydrogenase (Catalog #AAA849774) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849774 Assay Kit recognizes Eukaryotes Sorbitol Dehydrogenase. It is sometimes possible for the material contained within the vial of "Sorbitol Dehydrogenase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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