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Testing Data
General Phosphatidylinositol Assay Kit
PicoProbe Phosphatidylinositol Assay Kit (Fluorometric)
Reactivity
General
Synonyms
Phosphatidylinositol; PicoProbe Phosphatidylinositol Assay Kit (Fluorometric); Phosphoinositides; Phospholipid; Phosphatidylinositol assay kit
Reactivity
General
Samples
Tissue and cell lysates
Biological fluids (e.g.serum, plasma)
Biological fluids (e.g.serum, plasma)
Detection Method
Fluorometric: Ex/Em 535/587nm
Features & Benefits
The first kit in the market for the detection of Phosphatidylinositol.
This assay provides a convenient and non-ELISA alternative to detect cardiolipin amount in biological samples.
Measurement of PI content of various tissue/cell extracts Determination of PI concentration in biological fluids
This assay provides a convenient and non-ELISA alternative to detect cardiolipin amount in biological samples.
Measurement of PI content of various tissue/cell extracts Determination of PI concentration in biological fluids
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
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Related Product Information for Phosphatidylinositol assay kit
Phosphoinositides are critical second messengers in a multitude of cellular processes. The most fundamental phosphoinositide, phosphatidylinositol (PI), can be phosphorylated at multiple sites around its inositol ring, leading to downstream activation. For instance, phosphatidylinositol 4-[mono]phosphate is involved in membrane trafficking, while cleavage of phosphatidylinositol triphosphate yields inositol triphosphate, a molecule involved in calcium signaling. Signaling is conveyed in many signaling proteins (including Protein Kinase B/Akt) via their domain referred to as a Pleckstrin homology, or PH domain, which is capable of binding to specific phosphoinositides. Biosynthesis of PI involves the action of phosphatidylinositol synthase on cytidine diacylglycerol and myo-inositol. The critical signal transducer enzymes, Phosphatidylinositolinositol-3-kinases phosphorylate PI at the 3 position hydroxyl of inositol, leading to modulation in pathways such as cell growth, survival, and motility. PI is typically at concentrations in the low- to mid-micromolar range in human serum and plasma, and is capable of activating second messengers such as Ca2+ and cyclic AMP. It is through these pathways that PI has been found to affect such biological events as the pro-inflammatory response and intestinal uptake. Phosphatidylinositol Assay Kit utilizes specific enzymes to generate an intermediate that then reacts with PicoProbe, yielding a signal that can be quantified fluorometrically, and is proportional to the amount of PI present in the sample. The assay is capable of detecting as little as 15 pmoles of phosphatidylinositol.
Product Categories/Family for Phosphatidylinositol assay kit
Similar Products
Product Notes
The General Phosphatidylinositol (Catalog #AAA849759) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849759 Assay Kit recognizes General Phosphatidylinositol. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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