Mouse anti-Human MEF2A / MEF2 Monoclonal Antibody | anti-MEF2A antibody

Mouse Monoclonal [clone 1A10] (IgG2a,k) to Human MEF2A / MEF2

Cat. Num. AAA246042

• Gene Names: MEF2A; mef2; ADCAD1; RSRFC4; RSRFC9
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: MEF2A / MEF2; Monoclonal Antibody; Mouse Monoclonal [clone 1A10] (IgG2a; k) to Human MEF2A / MEF2; Anti-MEF2A / MEF2 Antibody (clone 1A10) IHC-plus; MEF2A; ADCAD1; RSRFC9; Mef2; Myocyte enhancer factor 2A; RSRFC4; Human MEF2A; MEF2; anti-MEF2A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1A10
• Specificity: Human MEF2A
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 499

• Applicable Applications for anti-MEF2A antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (2.5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western blot using tissue lysate and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: MEF2A (AAH13437, 71 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: MEF2A / MEF2 antibody was raised against mEF2A (AAH13437, 71 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-MEF2A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to MEF2A on HeLa cell. [antibody concentration 10 ug/ml].)

Western Blot (WB)

(MEF2A monoclonal antibody clone 1A10. Western blot of MEF2A expression in human lung cancer.)

ELISA (EIA)

(Detection limit for recombinant GST tagged MEF2A is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 195972799
• NCBI GeneID: 4205
• NCBI Accession #: NP_005578.2
• NCBI GenBank Nucleotide #: NM_005587.2
• UniProt Accession #: Q02078; O43814; Q14223; Q14224; Q59GX4; Q7Z6C9; Q96D14; B4DFQ7; F6XG23
• Molecular Weight: 45,570 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2A isoform 1
• NCBI Official Synonym Full Names: myocyte enhancer factor 2A
• NCBI Official Symbol: MEF2A
• NCBI Official Synonym Symbols: mef2; ADCAD1; RSRFC4; RSRFC9
• NCBI Protein Information: myocyte-specific enhancer factor 2A; serum response factor-like protein 1; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A)
• UniProt Protein Name: Myocyte-specific enhancer factor 2A
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2A
• UniProt Synonym Gene Names: MEF2
• UniProt Entry Name: MEF2A_HUMAN

NCBI Description

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

MEF2A: a myocyte-specific enhancing transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many, if not all, muscle-specific genes. A member of the MADS gene family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain. Five alternatively spliced isoforms have been reported.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein binding; transcription activator binding; protein heterodimerization activity; sequence-specific DNA binding; histone deacetylase binding; chromatin binding; SMAD binding; transcription factor activity; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; muscle development; ventricular cardiac myofibril development; nerve growth factor receptor signaling pathway; apoptosis; heart development; stress-activated MAPK cascade; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; mitochondrial genome maintenance; toll-like receptor 4 signaling pathway; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; dendrite morphogenesis; MAPKKK cascade; toll-like receptor 2 signaling pathway; mitochondrion distribution; MyD88-dependent toll-like receptor signaling pathway; muscle cell differentiation; toll-like receptor signaling pathway; innate immune response; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway

Disease: Coronary Artery Disease, Autosomal Dominant, 1