Testing Data
Actin Polymerization/Depolymerization Assay Kit
Actin Polymerization/Depolymerization Assay Kit (Fluorometric)
Synonyms
Actin Polymerization/Depolymerization; Actin Polymerization/Depolymerization Assay Kit (Fluorometric); Actin Polymerization/Depolymerization assay kit
Samples
Protein, Tissue Extracts, Compounds/Chemotherapeutic Agents
Detection Method
Fluorescence: Ex/Em 365/410nm
Features & Benefits
Study and quantitate the effect of different compounds, proteins and tissue extracts on Actin polymerization and/or depolymerization
Evaluation of critical concentrations of actin polymerization in different conditions
Evaluation of critical concentrations of actin polymerization in different conditions
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Actin Polymerization/Depolymerization assay kit
Actin, a highly conserved and abundant protein in eukaryotic cells, is one of the major components of cytoskeleton. It can be found as monomeric globular protein, called G actin or it can polymerize into filamentous actin, named F actin. Actin plays major roles in cell division, cell motility, cell signaling, organelle movement, etc. Mammals have 6 isoforms of Actin, which can be divided into 3 classes, alpha, beta and gamma. Muscle Actin is alpha class and all other non-muscle actins belong to beta and gamma -classes. Understanding the effect of different drugs, proteins, etc. on Actin Polymerization and Depolymerization is very important for understanding cellular machinery, more importantly because Cytoskeleton is a very important target for cancer therapy. Actin Polymerization/Depolymerization Assay Kit can be used to study the effect of different compounds, proteins and tissue extracts on Actin polymerization and depolymerization. The kit utilizes a proprietary Pyrene-labeled Actin molecule that develops a higher fluorescent signal if it undergoes polymerization. The signal can be easily detected using a fluorescence microplate reader. The assay is simple, high- throughput compatible, and can be completed in less than three hours
Product Categories/Family for Actin Polymerization/Depolymerization assay kit
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Product Notes
The Actin Polymerization/Depolymerization (Catalog #AAA849679) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Actin Polymerization/Depolymerization, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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