Typical Testing Data/Standard Curve (for reference only)
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Mammalian Salicylate Assay Kit
Salicylate Assay Kit (Colorimetric)
Reactivity
Mammalian
Synonyms
Salicylate; Salicylate Assay Kit (Colorimetric); Aspirin; Salicylic Acid; Salicylate assay kit
Reactivity
Mammalian
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for Salicylate assay kit
Background/Introduction: Salicylates (salts of 2-hydroxybenzoic acid) are common non-steroidal anti-inflammatory drugs (NSAIDs) with anti-pyretic and mild analgesic effects. Originally discovered in willow tree bark, salicylate has been used in an ethnopharmacological context for millennia to treat inflammation and fever. The most frequently used modern salicylate drug is aspirin (acetylsalicylic acid), which is rapidly hydrolyzed to salicylate in both the gastrointestinal tract and bloodstream. Salicylate acts as a weak inhibitor of the proinflammatory COX-2 enzyme, which is thought to underlie its anti-inflammatory effect in chronic aspirin therapy. However, supratherapeutic blood salicylate levels can cause severe intoxication and poisoning. Serum salicylate levels are monitored in people taking chronic high-dose aspirin for arthritis and in suspected cases of aspirin overdose. Therapeutic serum levels for salicylate range from 50-250 ug/ml (0.36 - 1.8 mM) in individuals on chronic aspirin therapy. Levels over 300 ug/ml (2.17 mM) are considered toxic, resulting in dose-dependent and potentially lethal symptoms such as tinnitus/deafness, lethargy/coma, seizures and metabolic acidosis. Salicylate Assay Kit is a high-throughput adaptable microplate-based assay that allows for rapid quantification of salicylate levels in biological fluids. In our assay, salicylate is enzymatically metabolized to catechol, with concomitant oxidation of a cofactor, resulting in a decrease in absorbance at OD = 405 nm that is proportional to the concentration of salicylate present. The assay is not affected by other NSAIDs and has an LOQ of 0.19 mM with a reliable linear range from 1 - 20 nmole salicylate per well (corresponding to 0.2 - 4 mM salicylate in undiluted samples).
Product Categories/Family for Salicylate assay kit
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Product Notes
The Mammalian Salicylate (Catalog #AAA849707) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849707 Assay Kit recognizes Mammalian Salicylate. It is sometimes possible for the material contained within the vial of "Salicylate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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