General LDL Uptake Assay Kit

LDL Uptake Assay Kit (Cell-Based)

Cat. Num. AAA849660

• Reactivity: General
• Synonyms: LDL Uptake; LDL Uptake Assay Kit (Cell-Based); LDL; LDL Uptake assay kit

• Reactivity: General

• Samples: Adherent cell line known to uptake LDL (e.g.HepG2 or Huh-7cells)
• Detection Method: Fluorometric: Ex/Em 488/523nm
• Features & Benefits: Measure LDL uptake in different cell types; Non-Radioactive; Contains fluorescent human LDL, which is covalently-labeled with a bright, highly photostable fluorophore,Screen, study or characterize stimulators or inhibitors of LDL uptake; Screening and characterization of drugs or treatment conditions that inhibit or stimulate LDL uptake
• Preparation and Storage: Store at -20 degree C; Shelf life: ~12 months

Immunocytochemistry/Immunofluorescence (ICC/IF))

Testing Data

Testing Data

Related Product Information for LDL Uptake assay kit

Low-density lipoprotein (LDL) is a large spherical macromolecule that serves as a 'shuttle' to transport hydrophobic molecules (lipids and cholesterol) throughout the body via the aqueous environment of the bloodstream. An LDL particle consists of a hydrophobic core of cholesterol esters (with long-chain fatty acids linked to the hydroxyl group) and triglycerides surrounded by an amphipathic shell containing the 510 kDa protein Apolipoprotein B-100, various phospholipids and non-esterified cholesterol molecules. LDL is taken up by cells such as hepatocytes, macrophages, and endothelial cells by specific receptor-mediated endocytosis. High levels of LDL have been associated with arterial inflammation, which leads to cardiovascular diseases (CVD) like atherosclerosis, ischemic stroke and myocardial infarction. LDL particles can migrate through inflamed vascular endothelial tight junctions, where they become oxidized and attract circulating monocytes. Monocytes differentiate into activated macrophages, which further accumulate LDL and become foam cells, leading to arterial wall thickening/scaring and formation of fibrous fatty plaques. Current CVD preventative therapies include decreasing LDL with statins, which prevent endogenous cholesterol biosynthesis by inhibiting HMG-CoA reductase. However, drugs that inhibit LDL uptake by macrophages or enhance LDL clearance by increasing hepatic LDL reuptake are also being investigated. LDL Uptake Assay Kit enables measurement of receptor-mediated LDL uptake by various cell types. The kit contains fluorescent human LDL, which is covalently-labeled with a bright, highly photostable fluorophore that can be detected when taken up by cells (Ex/Em = 488/523 nm). Unlabeled human LDL is included in the kit for validation of specific labeled LDL uptake. The kit contains a complete set of reagents sufficient for performing 100 reactions and a 96-well polylysine-coated black cell culture plate with a thin, microscopy-grade clear bottom.

Product Categories/Family for LDL Uptake assay kit

Cardiovascular Research; Ischemia Reperfusion Injury; Assay Kits; Atherosclerosis; Hypertension; Cancer Research; Angiogenesis; Diabetes; Obesity