Typical Testing Data/Standard Curve (for reference only)
Eukaryotes Cytochrome P450 Reductase (CPR) Assay Kit
Cytochrome P450 Reductase (CPR) Activity Kit (Colorimetric)
Reactivity
Eukaryotes
Synonyms
Cytochrome P450 Reductase (CPR); Cytochrome P450 Reductase (CPR) Activity Kit (Colorimetric); Cytochrome P450 Reductase (CPR) assay kit
Reactivity
Eukaryotes
Application Notes
The kit can detect CPR activity in a variety of sample types with a detection limit of ~0.2 mU per well.
Samples
Purified recombinant or native CPR protein
Human and animal liver microsomes
Lysates of tissues and cultured eukaryotic cells
Human and animal liver microsomes
Lysates of tissues and cultured eukaryotic cells
Detection Method
Absorbance (460 nm)
Features & Benefits
Only 96-well plate format CPR assay kit on the market
Only kit to use a synthetic colorimetric probe and a NADPH generating system
Rapid, convenient and sensitive
Kit contains all necessary reagents for accurate measurement of CPR activity down to 0.2 mU per well in a wide variety of sample types
Only kit to use a synthetic colorimetric probe and a NADPH generating system
Rapid, convenient and sensitive
Kit contains all necessary reagents for accurate measurement of CPR activity down to 0.2 mU per well in a wide variety of sample types
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cytochrome P450 Reductase (CPR) assay kit
Product Information: Cytochrome P450 Reductase (CPR) Activity Kit (Colorimetric): Quantitative 96-well plate assay of cytochrome P450 reductase activity in tissues, cells, microsomes or purified CPR enzyme. Simple & Sensitive. Detection limit: ~0.2 mU per well. 100 wells.
Background: NADPH-cytochrome P450 reductase (CPR, EC 1.6.2.4) is a ~78 kDa membrane-bound flavoenzyme that catalyzes the transfer of electrons from NADPH to members of the cytochrome P450 monooxidase (CYP) enzyme family in the endoplasmic reticulum. CPR contains two tightly bound flavin cofactors, FAD and FMN, which participate in the sequential transfer of electrons from NADPH-->FAD-->FMN-->CYP, oxidizing NADPH to NADP+ and reducing the CYP heme moiety to the substrate- and oxygen-binding ferrous state. As CPR is required for the function of all CYP isozymes, it plays a critical role in the metabolism of drugs, organic pollutants and other xenobiotic compounds, in addition to its role in biosynthesis of certain vitamins and steroid hormones. MyBioSource's cytochrome P450 reductase activity assay kit couples oxidation of NADPH by CPR to reduction of a nearly colorless probe into a brightly colored product with an absorbance peak at 460 nm, with the rate of color generation being directly proportional to CPR activity. The NADPH utilized by CPR is generated in situ from beta-NADP+ via oxidation of glucose-6-phosphate (G6P) to 6-phospho-D-glucono-1,5-lactone by glucose-6-phosphatase dehydrogenase (G6PDH). The kit can be used to determine CPR activity in a variety of samples, with a detection limit of ~0.2 mU of CPR activity per reaction. For assessment of CPR activity in crude biological samples that may have extraneous reductases capable of reducing the substrate, an inhibitor of NADPH-dependent flavoproteins is included. In this case, the specific CPR activity may be calculated by running parallel reactions in the presence and absence of inhibitor and subtracting any residual activity detected with the inhibitor present. The kit contains sufficient reagents for performing 100 reactions in a 96-well plate format.
Summary: Detection method- Absorbance (460 nm)
Species reactivity- Eukaryotes
Application- The kit can detect CPR activity in a variety of sample types with a detection limit of ~0.2 mU per well.
Background: NADPH-cytochrome P450 reductase (CPR, EC 1.6.2.4) is a ~78 kDa membrane-bound flavoenzyme that catalyzes the transfer of electrons from NADPH to members of the cytochrome P450 monooxidase (CYP) enzyme family in the endoplasmic reticulum. CPR contains two tightly bound flavin cofactors, FAD and FMN, which participate in the sequential transfer of electrons from NADPH-->FAD-->FMN-->CYP, oxidizing NADPH to NADP+ and reducing the CYP heme moiety to the substrate- and oxygen-binding ferrous state. As CPR is required for the function of all CYP isozymes, it plays a critical role in the metabolism of drugs, organic pollutants and other xenobiotic compounds, in addition to its role in biosynthesis of certain vitamins and steroid hormones. MyBioSource's cytochrome P450 reductase activity assay kit couples oxidation of NADPH by CPR to reduction of a nearly colorless probe into a brightly colored product with an absorbance peak at 460 nm, with the rate of color generation being directly proportional to CPR activity. The NADPH utilized by CPR is generated in situ from beta-NADP+ via oxidation of glucose-6-phosphate (G6P) to 6-phospho-D-glucono-1,5-lactone by glucose-6-phosphatase dehydrogenase (G6PDH). The kit can be used to determine CPR activity in a variety of samples, with a detection limit of ~0.2 mU of CPR activity per reaction. For assessment of CPR activity in crude biological samples that may have extraneous reductases capable of reducing the substrate, an inhibitor of NADPH-dependent flavoproteins is included. In this case, the specific CPR activity may be calculated by running parallel reactions in the presence and absence of inhibitor and subtracting any residual activity detected with the inhibitor present. The kit contains sufficient reagents for performing 100 reactions in a 96-well plate format.
Summary: Detection method- Absorbance (460 nm)
Species reactivity- Eukaryotes
Application- The kit can detect CPR activity in a variety of sample types with a detection limit of ~0.2 mU per well.
Product Categories/Family for Cytochrome P450 Reductase (CPR) assay kit
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Product Notes
The Eukaryotes Cytochrome P450 Reductase (CPR) (Catalog #AAA841584) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA841584 Assay Kit recognizes Eukaryotes Cytochrome P450 Reductase (CPR). The kit can detect CPR activity in a variety of sample types with a detection limit of ~0.2 mU per well. Researchers should empirically determine the suitability of the Cytochrome P450 Reductase (CPR) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 Reductase (CPR), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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