Typical Testing Data/Standard Curve (for reference only)
Succinyl-CoA Synthetase Assay Kit | SUCLA2 assay kit
Succinyl-CoA Synthetase Activity Colorimetric Assay Kit
Analysis of cell signaling pathway
Purified mitochondria
Cell culture: adherent or suspension cells
High-Throughput adaptable
This kit can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: Succinyl-CoA Synthetase (SCS, also called Succinyl-CoA ligase, Succinate Thiokinase) (EC 6.2.1.5) is a critical enzyme in the citric acid cycle and an important metabolic intermediate for porphyrin, heme and ketone body biosynthesis. It is located in the mitochondrial matrix and is a heterodimer composed of one alpha and one beta subunit. In humans, Succinyl-CoA Synthetase deficiency causes the build-up of lactic acid leading to lactic acidosis, which can be fatal in infants. Measurement and analysis of SCS activity is useful for both mechanistic studies as well as for purposes. In MyBioSource's Succinyl-CoA Synthetase Activity Assay, SCS converts succinate into succinyl-CoA in the presence of ATP and CoA. Succinyl-CoA reacts with the Developer to form a colored product with strong absorbance at 450 nm. This assay kit is simple, sensitive, and high-throughput adaptable. It can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.
Summary: Detection method- Absorbance (OD 450 nm).
Applications-
Measurement of Succinyl-CoA Synthetase activity in various tissues/cells
Analysis of cell signaling pathway
NCBI and Uniprot Product Information
NCBI Description
Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq, Jul 2008]
Uniprot Description
SCS-beta: a mitochondrial enzyme that catalyzes the phosphorylation of NTP, where N = adenosine or guanosine, in the tricarboxylic acid cycle. Catalyzes the following: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate. Two splice variant isoforms have been described.
Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; EC 6.2.1.5; Carbohydrate Metabolism - propanoate; Ligase
Chromosomal Location of Human Ortholog: 13q14.2
Cellular Component: mitochondrion; mitochondrial matrix
Molecular Function: protein binding; metal ion binding; succinate-CoA ligase (ADP-forming) activity; ATP binding
Biological Process: succinate metabolic process; cellular metabolic process; succinyl-CoA pathway; succinyl-CoA metabolic process; tricarboxylic acid cycle
Disease: Mitochondrial Dna Depletion Syndrome 5 (encephalomyopathic With Or Without Methylmalonic Aciduria)
Research Articles on SUCLA2
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Product Notes
The SUCLA2 sucla2 (Catalog #AAA841504) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of Succinyl-CoA Synthetase activity in various tissues/cells Analysis of cell signaling pathway. Researchers should empirically determine the suitability of the SUCLA2 sucla2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Succinyl-CoA Synthetase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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