Typical Testing Data/Standard Curve (for reference only)
Alpha-Ketoglutarate Dehydrogenase Assay Kit | KGD1 assay kit
Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit
Synonyms
Alpha-Ketoglutarate Dehydrogenase; Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit; KGD1 assay kit
Sequence Length
1015
Application Notes
Measurement of alpha-Ketoglutarate dehydrogenase activity in various tissues/cells
Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types
Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types
Samples
Animal tissues: liver, heart, muscle, etc.
Purified mitochondria
Cell culture: Adherent or suspension cells
Purified mitochondria
Cell culture: Adherent or suspension cells
Detection Method
Absorbance (450 nm)
Features & Benefits
Simple, rapid & convenient
can measure alpha-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples
can measure alpha-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for KGD1 assay kit
Product Information: alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) Activity Colorimetric Assay Kit: Simple & convenient assay to measure alpha Ketoglutarate Dehydrogenase activity in animal tissues, cells & purified mitochondria. Detection limit: lower than 0.1 mU. 100 Assays.
Background: alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). alpha-KGDH converts alpha-ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer's disease. Recent studies show that alpha-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit. MyBioSource's alpha-KGDH assay kit provides a quick and easy way for monitoring alpha-KGDH activity in various samples. In the assay, alpha-KGDH converts alpha-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect alpha-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Summary: Detection method-Absorbance (450 nm)
Applications- - Measurement of alpha-Ketoglutarate dehydrogenase activity in various tissues/cells - Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types
Background: alpha-Ketoglutarate Dehydrogenase (alpha-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). alpha-KGDH converts alpha-ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer's disease. Recent studies show that alpha-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit. MyBioSource's alpha-KGDH assay kit provides a quick and easy way for monitoring alpha-KGDH activity in various samples. In the assay, alpha-KGDH converts alpha-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect alpha-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Summary: Detection method-Absorbance (450 nm)
Applications- - Measurement of alpha-Ketoglutarate dehydrogenase activity in various tissues/cells - Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types
Product Categories/Family for KGD1 assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
114,107 Da
NCBI Official Full Name
alpha-ketoglutarate dehydrogenase
UniProt Protein Name
Alpha-ketoglutarate dehydrogenase
UniProt Gene Name
KGD1
UniProt Entry Name
A3LZW8_PICST
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Product Notes
The Alpha-Ketoglutarate Dehydrogenase kgd1 (Catalog #AAA841653) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of alpha-Ketoglutarate dehydrogenase activity in various tissues/cells Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types. Researchers should empirically determine the suitability of the Alpha-Ketoglutarate Dehydrogenase kgd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-Ketoglutarate Dehydrogenase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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