Testing Data
Mammalian NADH Oxidase Assay Kit
NADH Oxidase Activity Assay Kit (Colorimetric)
Reactivity
Mammalian
Synonyms
NADH Oxidase; NADH Oxidase Activity Assay Kit (Colorimetric); NADH Oxidase assay kit
Reactivity
Mammalian
Samples
Animal tissues: Liver, muscle, heart etc
Cell culture: adherent or suspension cells
Cell culture: adherent or suspension cells
Detection Method
Absorbance: OD 600nm
Features & Benefits
Can be used to determine NOX activity in a variety of sample types with a detection limit of ~ 1 mU of NOX activity per reaction
Simple, Rapid
Analysis of ROS in various samples
Measurement of NADH oxidase activity in various tissues/cells
Mechanistic studies of various cancers
Screening anti-cancer drugs
Simple, Rapid
Analysis of ROS in various samples
Measurement of NADH oxidase activity in various tissues/cells
Mechanistic studies of various cancers
Screening anti-cancer drugs
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Related Product Information for NADH Oxidase assay kit
NADH oxidase (Nicotinamide Adenine Dinucleotide Phosphate Oxidase, NOX) is a flavoprotein that catalyzes the oxidation of NADH by molecular oxygen to yield NAD+. NOX is one of the well-known cancer biomarkers and is shown to be stimulated by growth factors and hormones. Several studies revealed the high activity of NADH oxidase in cancer patients as compared to the healthy volunteers. More importantly, inhibition of NOX correlates with the reduced cancer cell growth and induction of apoptosis. RNA interference targeting NOX expression in cancer cells effectively restored the non-cancerous phenotypes, further supporting the vital role of NOX in cancer progression. Furthermore, NOX is critical for the regeneration of NAD+, for use in glycolysis and for the reduction of oxygen thereby preventing the formation of damaging reactive oxygen species. This makes NADH oxidase an attractive therapeutic target. NADH Oxidase Activity Assay Kit couples the oxidation of NADH by NOX and the reduction of a colored substrate to a colorless product. This result in the decrease of absorbance measured at 600 nm. The kit can be used to determine NOX activity in a variety of sample types with a detection limit of ~ 1 mU of NOX activity per reaction.
Product Categories/Family for NADH Oxidase assay kit
Similar Products
Product Notes
The Mammalian NADH Oxidase (Catalog #AAA849602) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849602 Assay Kit recognizes Mammalian NADH Oxidase. It is sometimes possible for the material contained within the vial of "NADH Oxidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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