Typical Testing Data/Standard Curve (for reference only)
Malate Dehydrogenase Assay Kit | MDH assay kit
Malate Dehydrogenase Activity Colorimetric Assay Kit
Gene Names
MDH; malate dehydrogenase; MALATE DEHYDROGENASE; MDH
Synonyms
Malate Dehydrogenase; Malate Dehydrogenase Activity Colorimetric Assay Kit; MDH assay kit
Sequence Length
403
Application Notes
Measurement of malate dehydrogenase activity in various tissues/cells
Analysis of citric acid cycle and malate-asparate shuttle
Analysis of citric acid cycle and malate-asparate shuttle
Samples
Animal tissues such as liver, heart, muscle, etc.
Cell culture: Adherent or suspension cells
Mitochondria
Cell culture: Adherent or suspension cells
Mitochondria
Detection Method
Absorbance (450 nm)
Features & Benefits
Simple, rapid & convenient
The assay can detect Malate Dehydrogenase (MDH) Activity less than 0.5 mU in various sample types.
The assay can detect Malate Dehydrogenase (MDH) Activity less than 0.5 mU in various sample types.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MDH assay kit
Product Information: Malate Dehydrogenase (MDH) Activity Colorimetric Assay Kit: Highly-sensitive assay to measure Malate Dehydrogenase (MDH) activity in animal tissues, adherent or suspension cells, mitochondria etc. Detection limit: < 0.5 mU. 100 assay
Background: Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malate-aspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer's disease, and abnormal MDH activity in serum can serve as a tool for severe liver damage (e.g. Hepatocellular carcinoma). In MyBioSource's Malate Dehydrogenase Activity Assay kit, MDH reacts with malate to form an intermediate. The generated intermediate reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.
Summary: Detection method- Absorbance (450 nm)
Applications- - Measurement of malate dehydrogenase activity in various tissues/cells - Analysis of citric acid cycle and malate-asparate shuttle
Background: Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malate-aspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer's disease, and abnormal MDH activity in serum can serve as a tool for severe liver damage (e.g. Hepatocellular carcinoma). In MyBioSource's Malate Dehydrogenase Activity Assay kit, MDH reacts with malate to form an intermediate. The generated intermediate reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.
Summary: Detection method- Absorbance (450 nm)
Applications- - Measurement of malate dehydrogenase activity in various tissues/cells - Analysis of citric acid cycle and malate-asparate shuttle
Product Categories/Family for MDH assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,406 Da
NCBI Official Full Name
malate dehydrogenase
NCBI Official Symbol
MDH
NCBI Official Synonym Symbols
malate dehydrogenase; MALATE DEHYDROGENASE; MDH
NCBI Protein Information
malate dehydrogenase
UniProt Protein Name
Malate dehydrogenase, chloroplastic
Protein Family
UniProt Gene Name
At3g47520
UniProt Entry Name
MDHP_ARATH
NCBI Description
Encodes a protein with NAD-dependent malate dehydrogenase activity, located in chloroplasts.
Uniprot Description
Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments (Probable). Plays a key role in the metabolism of dark chloroplasts and non-green plastids. Essential for embryo viability (PubMed:24198233, PubMed:24453164). Plays an essential role in heterotrophic metabolism in embryos, and autotrophic metabolism in photosynthetic tissues as well (PubMed:24453164).
Research Articles on MDH
Similar Products
Product Notes
The MDH at3g47520 (Catalog #AAA841658) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of malate dehydrogenase activity in various tissues/cells Analysis of citric acid cycle and malate-asparate shuttle. Researchers should empirically determine the suitability of the MDH at3g47520 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Malate Dehydrogenase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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