Rabbit Cytochrome P450 Reductase Polyclonal Antibody | anti-CYPR antibody

Cytochrome P450 Reductase polyclonal antibody

Cat. Num. AAA565302

• Gene Names: Por; CYPOR
• Reactivity: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: Cytochrome P450 Reductase; Polyclonal Antibody; Cytochrome P450 Reductase polyclonal antibody; anti-CYPR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse, rat, bovine, dog, guinea pig, hamster, monkey, pig, rabbit and sheep Cytochrome P450 Reductase. Detects a band of ~78kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 678

• Applicable Applications for anti-CYPR antibody: Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Inhibition Assay
• Application Notes: Western Blot (1:2,000, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~78kDa by Western blot.
• Immunogen: Native rat Cytochrome p450 Reductase.
• Preparation and Storage: Long term storage: -20 degree C

Testing Data

(Lane 1: MWM, Lane 2: OSP-300, Lane 3: Human Liver Microsomes, Lane 4: Rat Liver Microsomes, Lane 5: Mouse Liver Microsomes, Lane 6: MWM)

Related Product Information for anti-CYPR antibody

Cytochrome p450 is a highly conserved and diverse family of heme-containing proteins that function in the metabolism of numerous endogenous and exogenous substrates.. The human genome encodes 57 p450 genes divided among 18 families, which together account for the diversity of substrates metabolized. p450 is the primary conjugating enzyme in phase I metabolism and detoxification of endogenous and exogenous compounds, and are primarily localized to the inner membranes of mitochondria and the ER. Eukaryotic microsomal cytochrome p450 enzymes receive electrons from the FAD- and FMN-containing enzyme NADPH:cytochrome p450 reductase (CPR).

Product Categories/Family for anti-CYPR antibody

Protein Synthesis & Folding; Oxidative Stress; Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 127966
• NCBI GeneID: 29441
• UniProt Accession #: P00388
• Molecular Weight: 76,963 Da
• NCBI Official Full Name: NADPH--cytochrome P450 reductase
• NCBI Official Synonym Full Names: P450 (cytochrome) oxidoreductase
• NCBI Official Symbol: Por
• NCBI Official Synonym Symbols: CYPOR
• NCBI Protein Information: NADPH--cytochrome P450 reductase
• UniProt Protein Name: NADPH--cytochrome P450 reductase
• UniProt Gene Name: Por
• UniProt Synonym Gene Names: CPR; P450R
• UniProt Entry Name: NCPR_RAT

NCBI Description

plays a role in electron transport [RGD, Feb 2006]

Uniprot Description

POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.

Protein type: Mitochondrial; Endoplasmic reticulum; Apoptosis; Hydrolase; Membrane protein, peripheral; Oxidoreductase; EC 1.6.2.4

Cellular Component: endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle; membrane; mitochondrion

Molecular Function: [methionine synthase] reductase activity; cytochrome-b5 reductase activity; electron carrier activity; enzyme binding; FAD binding; FMN binding; hydrolase activity; iron ion binding; iron-cytochrome-c reductase activity; NADP binding; NADPH-hemoprotein reductase activity; nitric oxide dioxygenase activity; oxidoreductase activity

Biological Process: carnitine metabolic process; fatty acid oxidation; flavonoid metabolic process; internal peptidyl-lysine acetylation; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of lipase activity; nitrate catabolic process; nitric oxide catabolic process; positive regulation of cholesterol biosynthetic process; positive regulation of chondrocyte differentiation; positive regulation of monooxygenase activity; positive regulation of smoothened signaling pathway; response to drug; response to nutrient