Typical Testing Data/Standard Curve (for reference only)
Glucose-1-Phosphate Assay Kit
Glucose-1-Phosphate Colorimetric Assay Kit
Synonyms
Glucose-1-Phosphate; Glucose-1-Phosphate Colorimetric Assay Kit; G1P Colorimetric Assay Kit; Glucose-1-Phosphate assay kit
Samples
Animal tissues: Liver, muscle and heart etc.
Cell culture: Adherent or suspension cells.
Cell culture: Adherent or suspension cells.
Features & Benefits
Simple procedure; takes ~ 40 minutes
Rapid, convenient and sensitive
Kit contains all necessary reagents for accurate measurement of glucose-1-phosphate levels
Rapid, convenient and sensitive
Kit contains all necessary reagents for accurate measurement of glucose-1-phosphate levels
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Glucose-1-Phosphate assay kit
Product Information: Glucose-1-Phosphate (G1P) Assay Kit: Quantitative Colorimetric G1P determination in cells and animal tissues. Rapid, Simple & Sensitive. 100 wells. Detection limit: 1uM to 10 mM.
Background: Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P ? G1P), UDPG-pyrophosphorylase (G1P ? UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] ? UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties. In Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. MyBioSource's Glucose-1-phosphate assay kit is rapid, sensitive & easy to use & can detect 1uM to 10 mM G1P. This G1P assay kit can be used for a variety of sample types.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Application- The kit can detect glucose-1-phosphate concentrations in the range of 1 uM - 10 mM.
Background: Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P ? G1P), UDPG-pyrophosphorylase (G1P ? UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] ? UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties. In Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. MyBioSource's Glucose-1-phosphate assay kit is rapid, sensitive & easy to use & can detect 1uM to 10 mM G1P. This G1P assay kit can be used for a variety of sample types.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Application- The kit can detect glucose-1-phosphate concentrations in the range of 1 uM - 10 mM.
Product Categories/Family for Glucose-1-Phosphate assay kit
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Product Notes
The Glucose-1-Phosphate (Catalog #AAA841711) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glucose-1-Phosphate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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