Typical Testing Data/Standard Curve (for reference only)
Gluconokinase (GntK) Assay Kit
Gluconokinase (GntK) Activity Assay Kit (Colorimetric)
Synonyms
Gluconokinase (GntK); Gluconokinase (GntK) Activity Assay Kit (Colorimetric); Gluconate Kinase Activity Assay Kit (Colorimetric); ATP:D-gluconate 6-phosphotransferase Activity Assay Kit (Colorimetric); Gluconokinase (GntK) assay kit
Sequence Length
517
Application Notes
Measurement of GntK activity in various samples
Mechanistic study of Pentose Phosphate Pathway
Mechanistic study of Pentose Phosphate Pathway
Samples
Prokaryote such as: E.coli
Animal tissues such as liver, kidney, etc.
Adherent or suspension cells.
Animal tissues such as liver, kidney, etc.
Adherent or suspension cells.
Detection Method
Absorbance (450 nm)
Features & Benefits
Simple, rapid & convenient
The assay can detect gluconokinase activity less than 0.1 mU in a variety of samples
The assay can detect gluconokinase activity less than 0.1 mU in a variety of samples
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Gluconokinase (GntK) assay kit
Product Information: Gluconokinase (Gntk) Activity Assay Kit (Colorimetric): Simple & convenient assay to measure Gluconate Kinase activity in animal tissues, adherent & suspension cells & Prokaryotes. Detection limit: < 0.1 mU. 100 assay
Background: Gluconokinase (GntK or ATP:D-gluconate 6-phosphotransferase or Gluconate Kinase; EC:2.7.1.12) is a key enzyme for Gluconate degradation pathway. In E. coli and yeast, Gluconokinase can convert gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. Through Hexose Monophosphate Shunt (HMS) pathway, 6-Phosphate-D-Gluconate generates ribose-6-phosphate, which is critical for nucleotides and nucleic acid synthesis. Little is known of the mechanism of gluconate metabolism in humans despite its widespread use in medicine and consumer products. MyBioSource's Gluconokinase Assay kit provides a quick and easy way for monitoring Gluconokinase activity in a variety of samples. In this kit, Gluconokinase converts Gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. 6-Phosphate-D-Gluconate and ADP in turn undergoe a series of reactions to form an intermediate, which reacts with the probe to form a colored product with strong absorbance (OD 450 nm). The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1mU.
Summary: Detection method- Absorbance (450 nm)
Applications- Measurement of GntK activity in various samples - Mechanistic study of Pentose Phosphate Pathway
Background: Gluconokinase (GntK or ATP:D-gluconate 6-phosphotransferase or Gluconate Kinase; EC:2.7.1.12) is a key enzyme for Gluconate degradation pathway. In E. coli and yeast, Gluconokinase can convert gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. Through Hexose Monophosphate Shunt (HMS) pathway, 6-Phosphate-D-Gluconate generates ribose-6-phosphate, which is critical for nucleotides and nucleic acid synthesis. Little is known of the mechanism of gluconate metabolism in humans despite its widespread use in medicine and consumer products. MyBioSource's Gluconokinase Assay kit provides a quick and easy way for monitoring Gluconokinase activity in a variety of samples. In this kit, Gluconokinase converts Gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. 6-Phosphate-D-Gluconate and ADP in turn undergoe a series of reactions to form an intermediate, which reacts with the probe to form a colored product with strong absorbance (OD 450 nm). The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1mU.
Summary: Detection method- Absorbance (450 nm)
Applications- Measurement of GntK activity in various samples - Mechanistic study of Pentose Phosphate Pathway
Product Categories/Family for Gluconokinase (GntK) assay kit
NCBI and Uniprot Product Information
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Product Notes
The Gluconokinase (GntK) (Catalog #AAA841660) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of GntK activity in various samples Mechanistic study of Pentose Phosphate Pathway. Researchers should empirically determine the suitability of the Gluconokinase (GntK) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gluconokinase (GntK), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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