Western Blot (WB)
(Western Blot (WB) analysis of p-GABAA Rbeta1 (S434) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:Rat brain tissue lysate)
Rabbit GABAA Rbeta1 Polyclonal Antibody | anti-GABAARbeta1 antibody
GABAA Rbeta1 (Phospho-S434) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
GABAA Rbeta1; Polyclonal Antibody; GABAA Rbeta1 (Phospho-S434) Polyclonal Antibody; Gamma-aminobutyric acid receptor subunit beta-1; GABA(A) receptor subunit beta-1; GABRB1; GABAARbeta1; anti-GABAARbeta1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
p-GABAA Rbeta1 (S434) polyclonal antibody detects endogenous levels of GABAA Rbeta1 protein when phosphorylated at Ser434.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
474
Applicable Applications for anti-GABAARbeta1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic phosphopeptide derived from human GABAA Rbeta1 around the phosphorylation site of Serine 434.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of p-GABAA Rbeta1 (S434) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:Rat brain tissue lysate)
Western Blot (WB)
(Western Blot (WB) analysis of p-GABAA Rbeta1 (S434) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:Rat brain tissue lysate)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of p-GABAA Rbeta1 (S434) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of p-GABAA Rbeta1 (S434) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)
Related Product Information for anti-GABAARbeta1 antibody
GAD-65 and GAD-67, glutamate decarboxylases, function to catalyze the production of GABA (gamma-aminobutyric acid). In the central nervous system GABA functions as the main inhibitory transmitter by increasing a Cl- conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. Both GABAA and GABAC are ligand-gated ion channels, however, they are structurally and functionally distinct. Members of the GABAA receptor family include GABAA Ralpha1-6, GABAA R beta1-3, GABAA Rgamma1-3, GABAA Rdelta, GABAA Repsilon, GABAA R?1 and GABAA R?2. The GABAB family is composed of GABAB R1alpha and GABAB R1beta. GABA transporters have also been identified and include GABA T-1, GABA T-2 and GABA T-3 (also designated GAT-1, -2, and -3). The GABA transporters function to terminate GABA action.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 54kDa
NCBI Official Full Name
gamma-aminobutyric acid receptor subunit beta-1
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit beta-1
UniProt Gene Name
GABRB1
UniProt Entry Name
GBRB1_HUMAN
Uniprot Description
GABRB1: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily.
Protein type: Channel, chloride; Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, ion channel; Channel, ligand-gated
Chromosomal Location of Human Ortholog: 4p12
Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction
Molecular Function: chloride channel activity; GABA-A receptor activity; ligand-gated ion channel activity; extracellular ligand-gated ion channel activity
Biological Process: synaptic transmission; transport; ion transport; signal transduction; transmembrane transport
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Product Notes
The GABAARbeta1 gabrb1 (Catalog #AAA3004628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GABAA Rbeta1 (Phospho-S434) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GABAA Rbeta1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GABAARbeta1 gabrb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABAA Rbeta1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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