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Quality Control (Western blot analysis of immunized recombinant protein, using anti-LMO1 monoclonal antibody. )

Mouse anti-Human LMO1 Monoclonal Antibody | anti-LMO1 antibody

Mouse monoclonal antibody Anti-Human LMO1

Gene Names
LMO1; TTG1; RBTN1; RHOM1
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry
Synonyms
LMO1; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human LMO1; Homo sapiens LIM domain only 1 (rhombotin 1); mRNA (cDNA clone MGC:116691 IMAGE:40000076); complete cds.; RHOM1; TTG1; anti-LMO1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
790C2a
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
663
Applicable Applications for anti-LMO1 antibody
Dot Blot (DB), Immunocytochemistry (ICC)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-LMO1 monoclonal antibody. )

Quality Control (Western blot analysis of immunized recombinant protein, using anti-LMO1 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LMO1 mAb. [Lot No.790C2a-1])

Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LMO1 mAb. [Lot No.790C2a-1])
Related Product Information for anti-LMO1 antibody
LMO1 enocdes a cysteine-rich, two LIM domain transcriptional regulator. It is mapped to an area of consistent chromosomal translocation in chromosome 11, disrupting it in T-cell leukemia, although more rarely than the related gene, LMO2 is disrupted.[NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
17,697 Da
NCBI Official Full Name
Homo sapiens LIM domain only 1 (rhombotin 1), mRNA
NCBI Official Synonym Full Names
LIM domain only 1 (rhombotin 1)
NCBI Official Symbol
LMO1
NCBI Official Synonym Symbols
TTG1; RBTN1; RHOM1
NCBI Protein Information
rhombotin-1; LIM domain only protein 1; LMO-1; T-cell translocation gene 1; T-cell translocation protein 1; cysteine-rich protein TTG-1
UniProt Protein Name
Rhombotin-1
Protein Family
UniProt Gene Name
LMO1
UniProt Entry Name
RBTN1_HUMAN

NCBI Description

This locus encodes a transcriptional regulator that contains two cysteine-rich LIM domains but lacks a DNA-binding domain. LIM domains may play a role in protein interactions; thus the encoded protein may regulate transcription by competitively binding to specific DNA-binding transcription factors. Alterations at this locus have been associated with acute lymphoblastic T-cell leukemia. Chromosomal rearrangements have been observed between this locus and at least two loci, the delta subunit of the T-cell antigen receptor gene and the LIM domain binding 1 gene. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2012]

Uniprot Description

LMO1: May be involved in gene regulation within neural lineage cells potentially by direct DNA binding or by binding to other transcription factors. A chromosomal aberration involving LMO1 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p15;q11) with TCRD.

Protein type: DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: nucleus

Molecular Function: zinc ion binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter

Research Articles on LMO1

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Product Notes

The LMO1 lmo1 (Catalog #AAA120420) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human LMO1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LMO1 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the LMO1 lmo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LMO1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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