Typical Testing Data/Standard Curve (for reference only)
Pyruvate Dehydrogenase (PDH) Assay Kit | aceF assay kit
Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit
Synonyms
Pyruvate Dehydrogenase (PDH); Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit; aceF assay kit
Sequence Length
629
Application Notes
Measurement of pyruvate dehydrogenase activity in various tissues/cells - Analysis of cell signaling pathway
Samples
Animal tissues: heart, liver, muscle, etc.
Purified mitochondria
Cell culture: Adherent or suspension cells
Purified mitochondria
Cell culture: Adherent or suspension cells
Detection Method
Absorbance (450 nm)
Features & Benefits
Simple, rapid & convenient
can measure pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples
can measure pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for aceF assay kit
Product Information: Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit: Simple & convenient assay to measure Pyruvate Dehydrogenase activity in animal tissues, cells & purified mitochondria. Detection limit: lower than 0.1 mU. 100 Assays.
Background: Pyruvate Dehydrogenase (PDH) (EC 1.2.4.1) has a vital role in carbohydrate metabolism. It forms a well-characterized enzyme complex with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). PDH converts pyruvate into acetyl-CoA in the presence of NAD and CoA, and links glycolysis to the citric acid cycle. PDH activity is inhibited by high intracellular ratios of ATP/ADP, NADH/NAD or Acetyl-CoA/CoA. In humans, PDH deficiency reduces mitochondrial function and is linked to neurodegenerative diseases. PDH deficiency is X-linked; it results in 2 forms of abnormality: a metabolic form (lactic acidosis) and a neurological form (seizure and/or neuropathological spasm). Recent studies show that PDH is a target of oncogene-induced senescence; activation of PDH enhances pyruvate utilization and increases respiration and redox stress. MyBioSource's PDH assay kit provides a quick and easy way for monitoring PDH activity in various samples. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Summary: Detection method- Absorbance (450 nm)
Applications- - Measurement of pyruvate dehydrogenase activity in various tissues/cells - Analysis of cell signaling pathway
Background: Pyruvate Dehydrogenase (PDH) (EC 1.2.4.1) has a vital role in carbohydrate metabolism. It forms a well-characterized enzyme complex with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). PDH converts pyruvate into acetyl-CoA in the presence of NAD and CoA, and links glycolysis to the citric acid cycle. PDH activity is inhibited by high intracellular ratios of ATP/ADP, NADH/NAD or Acetyl-CoA/CoA. In humans, PDH deficiency reduces mitochondrial function and is linked to neurodegenerative diseases. PDH deficiency is X-linked; it results in 2 forms of abnormality: a metabolic form (lactic acidosis) and a neurological form (seizure and/or neuropathological spasm). Recent studies show that PDH is a target of oncogene-induced senescence; activation of PDH enhances pyruvate utilization and increases respiration and redox stress. MyBioSource's PDH assay kit provides a quick and easy way for monitoring PDH activity in various samples. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Summary: Detection method- Absorbance (450 nm)
Applications- - Measurement of pyruvate dehydrogenase activity in various tissues/cells - Analysis of cell signaling pathway
Product Categories/Family for aceF assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
65,679 Da
NCBI Official Full Name
pyruvate dehydrogenase
UniProt Protein Name
Acetyltransferase component of pyruvate dehydrogenase complex
UniProt Gene Name
aceF
UniProt Entry Name
A0A0B2TAB5_PECCA
Uniprot Description
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.
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Product Notes
The Pyruvate Dehydrogenase (PDH) acef (Catalog #AAA841792) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of pyruvate dehydrogenase activity in various tissues/cells - Analysis of cell signaling pathway. Researchers should empirically determine the suitability of the Pyruvate Dehydrogenase (PDH) acef for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyruvate Dehydrogenase (PDH), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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